Use of RAPD to detect sodium arsenite-induced DNA damage in human lymphoblastoid cells

中山醫學大學機構典藏 CSMUIR > 醫學科技學院 > 生物醫學科學學系暨碩士班 > 期刊論文 > Item 310902500/18057

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题名:	Use of RAPD to detect sodium arsenite-induced DNA damage in human lymphoblastoid cells
作者:	Lee, Y.-C.;Yang, Vivian.C.;Wang, T.-S.
日期:	2007
上传时间:	2017-08-01T08:17:53Z (UTC)
摘要:	Inorganic arsenic is a known human carcinogen, yet its mechanism of action remains unclear. Our previous study showed that arsenite significantly induces oxidative DNA adducts and DNA-protein cross-links in several mammalian cell lines. In the present study, we used the random amplified polymorphic DNA (RAPD) assay to evaluate the possible target in the genomic DNA of human lymphoblastoid cells that were exposed to sodium arsenite. Treatment with both 10 and 80 μM arsenite for 4 h induced significant changes in RAPD profiles compared with the control pattern. Two 10-mer RAPD primers (D11 and F1) produced the most distinguishable banding profiles between arsenite-treated and control genomic DNA. The sequencing of four arsenite-sensitive RAPD bands showed that the RB1CC1 and PACE4 genes might be the DNA targets of sodium arsenite treatment. We propose that arsenite may induce sequence- or gene-specific damage and then change the RAPD profile in human lymphoblastoid cells. The results of our study also show that RAPD combined with other techniques is a good tool for detecting alterations in genomic DNA and for the direct screening of new molecular markers related to arsenite-induced carcinogenesis. © 2007 Elsevier Ireland Ltd. All rights reserved.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/18057
關聯:	Toxicology 239, 108-115
显示于类别:	[生物醫學科學學系暨碩士班] 期刊論文

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