中山醫學大學機構典藏 CSMUIR:Item 310902500/17960
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    题名: Epigallocatechingallate Inhibits Migration of Human Uveal Melanoma Cells via Downregulation of Matrix Metalloproteinase-2 Activity and ERK1/2 Pathway
    作者: Chang, Chi-Wu
    Hsieh, Yi-Hsien
    Yang, ei-En
    Yang, Shun-Fa
    Chen, Yueqin
    Hu, Dan-Ning
    贡献者: 中山醫大
    日期: 2014
    上传时间: 2017-07-11T09:01:25Z (UTC)
    ISSN: 2314-6133
    摘要: Abstract

    The effects of epigallocatechingallate (EGCG) on the migration and expression of MMP-2 of uveal melanoma cells have not been reported. We studied this effect and relevant signaling pathways in a human uveal melanoma cell line (M17). MTT study found that EGCG did not affect the cell viability of M17 cells up to 100 µM. Wound-healing assay showed that EGCG significantly reduced the migration of melanoma cells in a dose-dependent manner from 20 to 100 µM. Gelatin zymography showed that secreted MMP-2 activity was dose-dependently inhibited by EGCG, whereas the MMP-2 expression at protein and mRNA levels was not affected as determined by western blot and RT-PCR analysis. EGCG significantly increased the expressions of MMP-2 endogenous inhibitors (TIMP-2 and RECK) in M17 cells. Western blot analysis of MAPK signal pathways showed that EGCG significantly decreased phosphorylated ERK1/2 levels, but not p38 and JNK levels, in melanoma cells. ERK1/2 inhibitors also reduced the migration and activity of MMP-2 in M17 cells. The present study suggested EGCG at nontoxic levels could inhibit migration of melanoma cells via downregulation of activities of secreted MMP-2 through the inhibition of the ERK1/2 phosphorylation. Therefore, EGCG may be a promising agent to be explored for the prevention of metastasis of uveal melanoma.
    URI: http://dx.doi.org/10.1155/2014/141582
    https://ir.csmu.edu.tw:8080/ir/handle/310902500/17960
    關聯: BioMed Research International Volume 2014 (2014), Article ID 141582, 9 pages
    显示于类别:[醫學系] 期刊論文

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