Secondhand Smoke Exposure Enhances Cardiac Fibrosis Effects on the Aging Rat Hearts

中山醫學大學機構典藏 CSMUIR > 醫學院 > 醫學系 > 期刊論文 > Item 310902500/17905

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题名:	Secondhand Smoke Exposure Enhances Cardiac Fibrosis Effects on the Aging Rat Hearts
作者:	Wu, Jia-Ping;Shu, Nu Chang-Lee;Day, Cecilia Hsuan;Ho, Tsung-Jung;Viswanadha, Vijaya Padma;Chung, Li-Chin;Hwang, Jin-Ming;Jong, Gwo-Ping;Kuo, Wei-Wen;Huang, Chih-Yang
贡献者:	中山醫大
关键词:	Aging;Basic fibroblast growth factors;Connective tissue growth factor;Extracellular matrix;Secondhand smoke exposure
日期:	2016
上传时间:	2017-06-30T09:47:52Z (UTC)
摘要:	Abstract Background Examining aging rats exposed to secondhand smoke (SHS) engenders changes in left ventricular remodeling due to age- or disease-dependent alterations. Methods Rats were placed in whole-body exposure chambers and exposed to 10 cigarettes. Filtered air was introduced into the chamber at a low rate. Rats were exposed to SHS for 30 min, twice a day, 5 days per week for 1 month. After 4 weeks SHS exposure, rats were sacrificed for morphological study with trichome staining and left ventricular remodeling related protein analysis using western blot. Results Characteristic fibrotic morphology in the left ventricle increased significantly with aging and exposure to SHS. Exposure to SHS elevated TGFβ1/p-Smad2/3/CTGF and MMP2/MMP9 protein expression levels (p < 0.05). No significant differences in FGF-2 and UPA protein expression were noted as a result of SHS exposure. However, TIMP-1, TIMP-2, TIMP-3 and TIMP-4 protein expression were suppressed by SHS exposure. We also observed increased TGFβ1/p-Smad2/3/CTGF (p < 0.01), FGF-2/UPA (p < 0.05) and decreased TIMPs protein expression levels. Corresponding MMP2 and MMP9 upregulation occurred with aging and exposure to SHS. TGFβ1/p-Smad2/3/CTGF and FGF-2/UPA protein expression from SHS exposure were higher than that from aging. In contrast, MMP2 and MMP9 were increased in aging rats compared with SHS exposed rats (p < 0.05); however, TIMP-1 (p < 0.01), TIMP-2 (p < 0.01) and TIMP-3 (p < 0.05) were decreased. TIMP-4 protein expression levels were decreased compared with SHS exposed rats (p < 0.01). Conclusions Aging and SHS exposure in rats will produce elevated fibrosis. Exposure to SHS will accelerate aging and left ventricular fibrosis.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/17905
關聯:	Acta Cardiol Sin. 2016 Sep; 32(5): 594–603.
显示于类别:	[醫學系] 期刊論文

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