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https://ir.csmu.edu.tw:8080/ir/handle/310902500/17892
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| Title: | Evaluating the Bone Tissue Regeneration Capability of the Chinese Herbal Decoction Danggui Buxue Tang from a Molecular Biology Perspective |
| Authors: | Wang, Wen-Ling
Sheu, Shi-Yuan
Chen, Yueh-Sheng
Kao, Shung-Te
Fu, Yuan-Tsung
Kuo, Tzong-Fu
Chen, Kuo-Yu
Yao, Chun-Hsu |
| Contributors: | 中山醫大 |
| Date: | 2014 |
| Issue Date: | 2017-06-30T08:58:18Z (UTC)
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| ISSN: | 2314-6133 |
| Abstract: | Abstract
Large bone defects are a considerable challenge to reconstructive surgeons. Numerous traditional Chinese herbal medicines have been used to repair and regenerate bone tissue. This study investigated the bone regeneration potential of Danggui Buxue Tang (DBT), a Chinese herbal decoction prepared from Radix Astragali (RA) and Radix Angelicae Sinensis (RAS), from a molecular biology perspective. The optimal ratio of RA and RAS used in DBT for osteoblast culture was obtained by colorimetric and alkaline phosphatase (ALP) activity assays. Moreover, the optimal concentration of DBT for bone cell culture was also determined by colorimetric, ALP activity, nodule formation, Western blotting, wound-healing, and tartrate-resistant acid phosphatase activity assays. Consequently, the most appropriate weight ratio of RA to RAS for the proliferation and differentiation of osteoblasts was 5 : 1. Moreover, the most effective concentration of DBT was 1,000 μg/mL, which significantly increased the number of osteoblasts, intracellular ALP levels, and nodule numbers, while inhibiting osteoclast activity. Additionally, 1,000 μg/mL of DBT was able to stimulate p-ERK and p-JNK signal pathway. Therefore, DBT is highly promising for use in accelerating fracture healing in the middle or late healing periods. |
| URI: | http://dx.doi.org/10.1155/2014/853234
https://ir.csmu.edu.tw:8080/ir/handle/310902500/17892 |
| Relation: | BioMed Research International Volume 2014 (2014), Article ID 853234, 10 pages |
| Appears in Collections: | [醫學系] 期刊論文
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