Effectiveness of hypochlorous acid to reduce the biofilms on titanium alloy surfaces in vitro

doi:10.3390/ijms17071161

中山醫學大學機構典藏 CSMUIR > 口腔醫學院 > 口腔醫學研究所 > 期刊論文 > Item 310902500/15621

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/15621

Title:	Effectiveness of hypochlorous acid to reduce the biofilms on titanium alloy surfaces in vitro
Authors:	Chen, C.-J. Chen, C.-C. Ding, S.-J.
Contributors:	醫學研究所
Keywords:	Antimicrobial activity;Antiseptics;Dental implant;Hypochlorite acid
Date:	2016-06-19
Issue Date:	2016-08-04T04:20:53Z (UTC)
Publisher:	MDPI AG
ISSN:	1661-6596
Abstract:	Chemotherapeutic agents have been used as an adjunct to mechanical debridement for peri-implantitis treatment. The present in vitro study evaluated and compared the effectiveness of hypochlorous acid (HOCl), sodium hypochlorite (NaOCl), and chlorhexidine (CHX) at eliminating Gram-negative (E. coli and P. gingivalis) and Gram-positive (E. faecalis and S. sanguinis) bacteria. The effect of irrigating volume and exposure time on the antimicrobial efficacy of HOCl was evaluated, and a durability analysis was completed. Live/dead staining, morphology observation, alamarBlue assay, and lipopolysaccharide (LPS) detection were examined on grit-blasted and biofilm-contaminated titanium alloy discs after treatment with the three chemotherapeutic agents. The results indicated that HOCl exhibited better antibacterial efficacy with increasing irrigating volumes. HOCl achieved greater antibacterial efficacy as treatment time was increased. A decrease in antimicrobial effectiveness was observed when HOCl was unsealed and left in contact with the air. All the irrigants showed antibacterial activity and killed the majority of bacteria on the titanium alloy surfaces of biofilm-contaminated implants. Moreover, HOCl significantly lowered the LPS concentration of P. gingivalis when compared with NaOCl and CHX. Thus, a HOCl antiseptic may be effective for cleaning biofilm-contaminated implant surfaces. © 2016 by the authors; licensee MDPI, Basel, Switzerland.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/15621 http://dx.doi.org/10.3390/ijms17071161
Relation:	International Journal of Molecular Sciences Volume 17, Issue 7, 19 July 2016, 論文編號 1161
Appears in Collections:	[口腔醫學研究所] 期刊論文

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