中山醫學大學機構典藏 CSMUIR:Item 310902500/11924
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    請使用永久網址來引用或連結此文件: https://ir.csmu.edu.tw:8080/ir/handle/310902500/11924


    題名: Quantitative determination of urinary N3-methyladenine by isotope-dilution LC-MS/MS with automated solid-phase extraction
    作者: Chiung-Wen Hua;Bo-Huei Linc;Mu-Rong Chao
    貢獻者: 中山醫學大學
    關鍵詞: N3-methyladenine;DNA adducts;LC–MS/MS;On-line solid-phase extraction;Cigarette smoking
    日期: 2011-07
    上傳時間: 2015-08-04T10:31:09Z (UTC)
    摘要: A rapid, highly specific and sensitive liquid chromatographic–tandem mass spectrometric (LC–MS/MS) method was developed for measuring urinary N3-methyladenine (N3-MeA). With the use of an isotope internal standard (d3-N3-MeA) and on-line solid-phase extraction (SPE), urine samples can be directly analyzed within 12 min without prior sample purification. The detection limit of this method was estimated as 0.035 ng/mL on-column (11.7 fmol). Intra- and inter-day imprecision (CV) were <7.4% and <10.8%, respectively. Mean recovery of N3-MeA in urine was 97–101%. This method was further applied to study the methylating agents exposure arising from cigarette smoke. Eighty-six volunteers were recruited including 41 regular smokers and 45 nonsmokers. The results showed that the urinary levels of N3-MeA observed in smokers (10.9 ± 13.2 ng/mg creatinine) were significantly higher than those in nonsmokers (4.1 ± 4.3 ng/mg creatinine; P < 0.001). It was further noted that the urinary level of N3-MeA was found to be highly associated with cotinine for smokers (Spearman correlation coefficients, r = 0.69, P < 0.001), implying that cigarette smoking resulted in increased DNA methylation, followed by depurination and excretion of N3-MeA in urine. As a result of the on-line SPE system, this method is capable of routine high-throughput analysis and accurate quantification of N3-MeA, and would be a useful tool for the surveillance of methylating agent exposure and its associated cancer risk.
    URI: https://ir.csmu.edu.tw:8080/ir/handle/310902500/11924
    關聯: International Journal of Mass Spectrometry Volume 304, Issues 2–3, 1 ;68–73
    顯示於類別:[公共衛生學系暨碩士班] 期刊論文

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