Hedgehog overexpression leads to the formation of prostate cancer stem cells with metastatic property irrespective of androgen receptor expression in the mouse model

doi:10.1186/1423-0127-18-6

CSMUIR > The College of Medical Sciences and Technology > School of Medical Laboratory and Biotechnology > journal paper > Item 310902500/11864

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/11864

Title:	Hedgehog overexpression leads to the formation of prostate cancer stem cells with metastatic property irrespective of androgen receptor expression in the mouse model
Authors:	Chang, Han-Hsin Chen, Bo-Yie Wu, Chia-Yung Tsao, Zih-Jay Chen, Ying-Yu Chang, Chin-Pao Yang, Chi-Rei Lin, David Pei-Cheng
Contributors:	中山醫學大學
Date:	2011
Issue Date:	2015-07-30T09:26:00Z (UTC)
ISSN:	1021-7770
Abstract:	Background Hedgehog signalling has been implicated in prostate tumorigenesis in human subjects and mouse models, but its effects on transforming normal basal/stem cells toward malignant cancer stem cells remain poorly understood. Methods We produced pCX-shh-IG mice that overexpress Hedgehog protein persistently in adult prostates, allowing for elucidation of the mechanism during prostate cancer initiation and progression. Various markers were used to characterize and confirm the transformation of normal prostate basal/stem cells into malignant cancer stem cells under the influence of Hedgehog overexpression. Results The pCX-shh-IG mice developed prostatic intraepithelial neoplasia (PIN) that led to invasive and metastatic prostate cancers within 90 days. The prostate cancer was initiated through activation of P63+ basal/stem cells along with simultaneous activation of Hedgehog signalling members, suggesting that P63+/Patch1+ and P63+/Smo+ cells may serve as cancer-initiating cells and progress into malignant prostate cancer stem cells (PCSCs). In the hyperplastic lesions and tumors, the progeny of PCSCs differentiated into cells of basal-intermediate and intermediate-luminal characteristics, whereas rare ChgA+ neuroendocrine differentiation was seen. Furthermore, in the metastatic loci within lymph nodes, kidneys, and lungs, the P63+ PCSCs formed prostate-like glandular structures, characteristic of the primitive structures during early prostate development. Besides, androgen receptor (AR) expression was detected heterogeneously during tumor progression. The existence of P63+/AR-, CK14+/AR- and CD44+/AR- progeny indicates direct procurement of AR- malignant cancer trait. Conclusions These data support a cancer stem cell scenario in which Hedgehog signalling plays important roles in transforming normal prostate basal/stem cells into PCSCs and in the progression of PCSCs into metastatic tumor cells.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/11864 http://dx.doi.org/10.1186/1423-0127-18-6
Relation:	Journal of Biomedical Science 2011, 18:6
Appears in Collections:	[School of Medical Laboratory and Biotechnology] journal paper

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