Dietary α-lipoic acid prevents UVB-induced corneal and conjunctival degeneration through multiple effects.

doi:10.1167/iovs.12-10891

中山醫學大學機構典藏 CSMUIR > 醫學科技學院 > 醫學影像暨放射科學系暨碩士班 > 期刊論文 > Item 310902500/11856

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/11856

Title:	Dietary α-lipoic acid prevents UVB-induced corneal and conjunctival degeneration through multiple effects.
Authors:	Chen, BY Lin, DP Chang, LS Huang, TP Liu, HJ Luk, CP Lo, YL Chang, HH
Contributors:	中山醫學大學
Keywords:	UVB;photokeratitis;α-lipoic acid
Date:	2013
Issue Date:	2015-07-30T08:59:02Z (UTC)
ISSN:	0146-0404
Abstract:	PURPOSE: This study investigated the effects of dietary α-lipoic acid (α-LA) against ultraviolet B (UVB)-induced corneal and conjunctival degeneration in a mouse model. METHODS: Female CBA mice were randomly divided into five study groups, including blank control, UVB without α-LA, and UVB with dietary α-LA at 1, 10, and 100 mg/kg body weight. Following UVB exposure, corneal surfaces were assessed along with immunohistochemistry for nuclear factor-κB (NF-κB), cyclooxygenase-2 (COX-2), malondialdehyde (MDA) accumulation, and P63⁺ basal cell distribution. Matrix metalloproteinase (MMP)-2 and MMP-9 activities were determined by gelatin zymography. ELISA assay was performed to confirm the findings of immunohistochemistry for NF-κB, COX-2, and MDA, along with the levels of TNF-α and IL-6. Tear production and goblet cell density were determined after tear strip assay and periodic acid Schiff staining, respectively. RESULTS: The results showed that UVB irradiation caused corneal surface damage, polymorphonuclear leukocyte infiltration, and loss of P63⁺ basal cells. Dietary α-LA ameliorated the UVB-induced corneal damage while simultaneously reducing MDA accumulation and maintaining P63⁺ basal cell survival. NF-κB-p65, COX-2, TNF-α, IL-6, and MMP-9 activity were all reduced by dietary α-LA. In addition, α-LA helped to reverse aqueous tear reduction, conjunctival squamous epithelium metaplasia, and goblet cell loss after UVB exposure. CONCLUSIONS: Dietary α-LA can prevent UVB-induced corneal damage and can be used as a prophylactic agent prior to excessive UVB exposure.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/11856 http://dx.doi.org/10.1167/iovs.12-10891
Relation:	Invest Ophthalmol Vis Sci. 2013 Nov 5;54(10):6757-66.
Appears in Collections:	[醫學影像暨放射科學系暨碩士班] 期刊論文

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