The PKC delta inhibitor, rottlerin, induces apoptosis of haematopoietic cell lines through mitochondrial membrane depolarization and caspases' cascade.

doi:10.1016/j.lfs.2005.01.010

中山醫學大學機構典藏 CSMUIR > 醫學院 > 免疫學研究所 > 期刊論文 > Item 310902500/11724

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://ir.csmu.edu.tw:8080/ir/handle/310902500/11724

题名:	The PKC delta inhibitor, rottlerin, induces apoptosis of haematopoietic cell lines through mitochondrial membrane depolarization and caspases' cascade.
作者:	Liao, YF Hung, YC Chang, WH Tsay, GJ Hour, TC Hung, HC Liu, GY
贡献者:	中山醫學大學
关键词:	Mallotus philippinensis;Euphorbiaceae;Rottlerin;Apoptosis;Mitochondrial membrane potential;Cytochrome c;Caspase
日期:	2005
上传时间:	2015-07-28T07:56:00Z (UTC)
ISSN:	0024-3205
摘要:	Rottlerin is a widely selective protein kinase C delta (PKCdelta) inhibitor isolated from Mallotus philippinensis. It shown to be effective against several human tumor cell lines and in potentiating chemotherapy-induced cytotoxcicity. Using the trypan blue exclusion assay, we demonstrated that rottlerin reduced the viability in a dose- and time-dependent manner of human leukemia HL60 cells, human acute T cell leukemia Jurkat cells and mouse macrophage RAW 264.7 cells. Rottlerin caused apoptosis and the apaptotic processing was inhibited by a caspase inhibitor, z-VAD-fmk, in these haematopoietic cells. The apoptosis-inducing activities were determined by nuclear condensation, sub-G1 appearance, DNA fragmentation, loss of mitochondrial membrane potential (Deltapsim), release of mitochondrial cytochrome c into cytoplasm and proteolytic activation of caspase 9 and 3. Expression of PKCdelta and Bcl-2 protein inhibited Deltapsim change and repressed cell death. These studies suggest that the cytotoxic effects of rottlerin through inhibition of PKCdelta cause mitochondrial dysfunction, cytochrome c release from mitochondria into cytoplasm and the activation of caspases' cascade.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/11724 http://dx.doi.org/10.1016/j.lfs.2005.01.010
關聯:	Life Sci. 2005 Jun 24;77(6):707-19.
显示于类别:	[免疫學研究所] 期刊論文

文件中的档案:

档案	描述	大小	格式	浏览次数
index.html	期刊論文	0Kb	HTML	426	检视/开启

检视Licence

在CSMUIR中所有的数据项都受到原著作权保护.

TAIR相关文章

数据加载中.....