Rosiglitazone inhibits endothelial proliferation and angiogenesis.

doi:10.1016/j.lfs.2005.07.046

中山醫學大學機構典藏 CSMUIR > 醫學院 > 生化微生物免疫研究所 > 期刊論文 > Item 310902500/11577

請使用永久網址來引用或連結此文件: https://ir.csmu.edu.tw:8080/ir/handle/310902500/11577

題名:	Rosiglitazone inhibits endothelial proliferation and angiogenesis.
作者:	Sheu, WH Ou, HC Chou, FP Lin, TM Yang, CH
貢獻者:	中山醫學大學
關鍵詞:	Rosiglitazone;Cell cycle;Angiogenesis
日期:	2006
上傳時間:	2015-07-23T04:31:07Z (UTC)
ISSN:	0024-3205
摘要:	Rosiglitazone, an insulin sensitizer, is known to offer beneficial effects in retarding atherosclerotic vascular diseases. Since proliferation and angiogenesis are involved in initiation and plaque instability, two critical steps in the cardiovascular events, this study was designed to evaluate the mechanisms of rosiglitazone on endothelial proliferation and angiogenesis. Rosiglitazone-treated human umbilical vein endothelial cells were analyzed for growth rate by use of cell number counting, 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay as well as 3H-thymidine incorporation. Cell cycle analysis was detected by flow cytometry and cell cycle-related proteins were measured by Western blot. Effects of rosiglitazone on angiogenesis were assessed by vascular endothelial growth factor (VEGF)-induced tube formation and wound-healing migration. Furthermore, effects of rosiglitazone on actin stress fiber were observed under confocal microscopy. Our data showed that rosiglitazone inhibits endothelial proliferation in a dose-dependent manner. Rosiglitazone caused endothelial arrest at G1 phase via affecting several cell cycle-related proteins that led to attenuate phosphorylation of retinoblastoma protein. Rosiglitazone markedly decreased VEGF-induced tube formation and endothelial cell migration, which might be explained by a disorganization of the actin cytoskeleton. Our data suggest that both anti-proliferative and anti-angiogenic activities in endothelial cells might account for the greater than expected beneficial effects of rosiglitazone for the treatment and prevention of atherosclerosis.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/11577 http://dx.doi.org/10.1016/j.lfs.2005.07.046
關聯:	Life Sci. 2006 Feb 23;78(13):1520-8.
顯示於類別:	[生化微生物免疫研究所] 期刊論文

文件中的檔案:

檔案	描述	大小	格式	瀏覽次數
index.html	期刊論文	0Kb	HTML	347	檢視/開啟

檢視Licence

在CSMUIR中所有的資料項目都受到原著作權保護.

TAIR相關文章

資料載入中.....