Effects of nonsteroidal anti-inflammatory drugs on the expression of urokinase plasminogen activator and inhibitor and gelatinases in the early osteoarthritic knee of humans.

doi:10.1016/j.clinbiochem.2007.10.011

中山醫學大學機構典藏 CSMUIR > 醫學院 > 生化微生物免疫研究所 > 期刊論文 > Item 310902500/11480

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题名:	Effects of nonsteroidal anti-inflammatory drugs on the expression of urokinase plasminogen activator and inhibitor and gelatinases in the early osteoarthritic knee of humans.
作者:	Yang, SF Hsieh, YS Lue, KH Chu, SC Chang, IC Lu, KH
贡献者:	中山醫學大學
关键词:	NSAID;u-PA;PAI-1;MMP-2;MMP-9;Osteoarthritis;Cyclooxygenase-2
日期:	2008
上传时间:	2015-07-21T09:12:53Z (UTC)
ISSN:	0009-9120
摘要:	OBJECTIVES: The purpose of this study was to examine the ex vivo effects of nonsteroidal anti-inflammatory drugs (NSAIDs) on the expression of urokinase-type plasminogen activator (u-PA), PA inhibitor-1 (PAI-1) and gelatinases [matrix metalloproteinase (MMP)-2 and MMP-9] in the early knee osteoarthritis (OA) of humans. DESIGN AND METHODS: Samples of articular cartilage, meniscus and synovium of OA patients were obtained and cultured ex vivo in the presence or absence of NSAIDs (diclofenac sodium, nimesulide, celecoxib, valdecoxib, rofecoxib and etoricoxib). RESULTS: Gelatin zymography showed that all NSAIDs generally decreased MMP-2 secretion in chondral, meniscal and synovial cultures as well as MMP-9 production in meniscal and synovial cultures. ELISA showed the inhibitions of u-PA secretion in chondral cultures by diclofenac and rofecoxib as well as in chondral and synovial cultures by nimesulide, celecoxib and etoricoxib at 48 h. On PAI-1 secretion, rofecoxib in synovial cultures and diclofenac, nimesulide, celecoxib and etoricoxib in chondral and synovial cultures had significantly suppressive effects at 48 h. CONCLUSIONS: This study clearly demonstrates that NSAIDs can down-regulate the PA/plasmin system and gelatinases expression during the early stage of knee OA, thereby possibly affect the structural progression of the disease. This inhibition seems to be independent selection of COX-1 and COX-2.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/11480 http://dx.doi.org/10.1016/j.clinbiochem.2007.10.011
關聯:	Clin Biochem. 2008 Jan;41(1-2):109-16.
显示于类别:	[生化微生物免疫研究所] 期刊論文

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