Platelet-rich fibrin increases proliferation and differentiation of human dental pulp cells.

doi:10.1016/j.joen.2010.07.004

中山醫學大學機構典藏 CSMUIR > 口腔醫學院 > 牙醫學系暨碩士班 > 期刊論文 > Item 310902500/11377

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题名:	Platelet-rich fibrin increases proliferation and differentiation of human dental pulp cells.
作者:	Huang, FM Yang, SF Zhao, JH Chang, YC
贡献者:	中山醫學大學
日期:	2010
上传时间:	2015-07-16T09:36:08Z (UTC)
ISSN:	0099-2399
摘要:	INTRODUCTION: Platelet-rich fibrin (PRF) by Choukroun's technique is derived from an autogenous preparation of concentrated platelets without any manipulation. When delicately pressed between 2 gauzes, the PRF clot becomes a strong membrane with high potential in clinical application. However, the effect of PRF on dental pulp cells (DPCs) remains to be elucidated. This study was to determine the biological effects of PRF on DPCs. METHODS: PRF samples were obtained from 6 healthy volunteers. Human DPCs were derived from healthy individuals undergoing extraction for third molars. Cell proliferation resulting from PRF was evaluated by colorimetric assay. Western blot was used to evaluate the expression of osteoprotegerin (OPG). Alkaline phosphatase (ALP) activity was examined by substrate assay. RESULTS: PRF did not interfere with cell viability of DPCs (P > .05). DPCs were observed to attach at the edges of PRF by phase-contrast microscopy. PRF was found to increase DPC proliferation during 5-day incubation period (P < .05). PRF was found to increase OPG expression in a time-dependent manner (P < .05). ALP activity was also significantly up-regulated by PRF (P < .05). CONCLUSIONS: PRF was demonstrated to stimulate cell proliferation and differentiation of DPCs by up-regulating OPG and ALP expression. These findings might serve as a basis for preclinical studies that address the role of PRF in reparative dentin formation. Crown Copyright © 2010. Published by Elsevier Inc. All rights reserved.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/11377 http://dx.doi.org/10.1016/j.joen.2010.07.004
關聯:	J Endod. 2010 Oct;36(10):1628-32.
显示于类别:	[牙醫學系暨碩士班] 期刊論文

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