Cytotoxic effects of denture base materials on a permanent human oral epithelial cell line and on primary human oral fibroblasts in vitro.

中山醫學大學機構典藏 CSMUIR > 口腔醫學院 > 牙醫學系暨碩士班 > 期刊論文 > Item 310902500/11369

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题名:	Cytotoxic effects of denture base materials on a permanent human oral epithelial cell line and on primary human oral fibroblasts in vitro.
作者:	Huang, FM Tai, KW Hu, CC Chang, YC
贡献者:	中山醫學大學
日期:	2001
上传时间:	2015-07-16T09:16:45Z (UTC)
摘要:	PURPOSE: The objective of this study was to determine the cytocompatibility of three different extracts of denture base resins and to compare the cytotoxic effect of these materials on a human oral epithelial KB cell line and primary human oral fibroblasts derived from buccal mucosa. MATERIALS AND METHODS: Set specimens from a heat-cured resin, a self-cured resin, and a light-cured resin were eluted with culture medium for 1, 3, and 5 days. Cytotoxicity was judged using tetrazolium bromide reduction assay. RESULTS: The eluates from self-cured, heat-cured, and light-cured denture base resins were cytotoxic to primary human buccal fibroblast cultures and KB cells. Self-cured resin was the most toxic denture base material among the chemicals tested in all cultures. The cytotoxicity decreased in the order of self-cured resin > heat-cured resin > light-cured resin for KB cells. The rank for buccal fibroblast cells was self-cured resin > heat-cured resin > light-cured resin. CONCLUSION: The influence of the cytotoxicity depended on the materials tested and the cell culture system used. The use of both permanent and primary cells is recommended for a better screening of the cytotoxic effects of denture base resins.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/11369
關聯:	Int J Prosthodont. 2001 Sep-Oct;14(5):439-43.
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