中山醫學大學機構典藏 CSMUIR:Item 310902500/11338
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    题名: Regulation of nicotine-induced cyclooxygenase-2 protein expression in human gingival fibroblasts.
    作者: Ho, YC
    Chang, YC
    贡献者: 中山醫學大學
    日期: 2006
    上传时间: 2015-07-15T10:40:53Z (UTC)
    ISSN: 1671-4083
    摘要: AIM:
    Activation of cyclooxygenase-2 (COX-2) expression by nicotine suggests a potential role for nicotine in the pathogenesis of smoking-associated periodontal disease. The aim of this study was to investigate whether chemical interactions can modulate nicotine-induced COX-2 expression in human gingival fibroblasts (HGF).
    METHODS:
    Cytotoxicity was investigated by using lactate dehydrogenase leakage assays and Western blotting was used to assess COX-2 expression. Furthermore, buthionine sulfoximine (BSO; an intracellular glutathione synthesis inhibitor), 2-oxothiazolidine-4-carboxylic acid (OTZ; the precursor of cysteine), and PD98059 (extracellular signal-regulated protein kinase inhibitor) were added to search for the possible regulation mechanisms of nicotine-induced COX-2 expression.
    RESULTS:
    Nicotine was found to elevate lactate dehydrogenase leakage in a dose-dependent manner (P<0.05). Treatment of HGF with nicotine was shown to mediate COX-2 protein expression. Pretreatment with OTZ decreased nicotine-induced COX-2 protein level by approximately 60 % (P<0.05). However, BSO enhanced nicotine-induced COX-2 protein level up to approximately 3-fold (P<0.05). Treatment of HGF with PD98059 decreased nicotine-induced COX-2 protein expression. In addition, nicotine induced extracellular signal-regulated protein kinase phosphorylation in a time-dependent manner (P<0.05).
    CONCLUSION:
    Nicotine may play a significant role in the pathogenesis of cigarette smoking associated-periodontitis via the activation of COX-2 which is augmented by oxidative stress and mediated by extracellular signal-regulated protein kinase signaling.
    URI: https://ir.csmu.edu.tw:8080/ir/handle/310902500/11338
    http://dx.doi.org/10.1111/j.1745-7254.2006.00286.x
    關聯: Acta Pharmacol Sin. 2006 Apr;27(4):409-13.
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