Effects of a bacterial lipid byproduct on human pulp fibroblasts in vitro.

doi:10.1016/j.joen.2006.12.022

中山醫學大學機構典藏 CSMUIR > 口腔醫學院 > 牙醫學系暨碩士班 > 期刊論文 > Item 310902500/11331

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题名:	Effects of a bacterial lipid byproduct on human pulp fibroblasts in vitro.
作者:	Ho, YC Chang, YC
贡献者:	中山醫學大學 http://dx.doi.org/10.1016/j.joen.2006.12.022
日期:	2007
上传时间:	2015-07-15T10:21:05Z (UTC)
ISSN:	0099-2399
摘要:	Butyrate, a short chain fatty acid, is a metabolic lipid byproduct of various root canal pathogens, such as Porphyromonas endodontalis. However, little is known about the effects of butyrate on cultured human pulp fibroblasts. H33258 fluorescence, flow cytometry, and protein synthesis assays were used to investigate the pathobiologic effects of butyrate on cultured human pulp fibroblasts. Butyrate exhibited cytotoxic effects on human pulp fibroblasts in a concentration-dependent manner (p < 0.05). The addition of butyrate resulted in G2/M phase arrest (p < 0.05). Butyrate also inhibited protein synthesis in a dose-dependent manner (p < 0.05). To determine whether glutathione (GSH) levels were important in the cytotoxicity of butyrate, we pretreated cells with the GSH precursor, 2-oxothiazolidine-4-carboxylic acid (OTZ), to boost thiol levels, or buthionine sulfoximine (BSO) to deplete GSH. The addition of OTZ acted as a protective effect on the butyrate-induced cytotoxicity (p < 0.05). In contrast, the addition of BSO enhanced the butyrate-induced cytotoxicity (p < 0.05). These results indicate that butyrate is cytotoxic to human pulp fibroblasts by inhibiting cell growth, cell-cycle kinetics, and protein synthesis. These inhibitory effects were associated with intracellular GSH levels.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/11331
關聯:	J Endod. 2007 Apr;33(4):437-41. Epub 2007 Feb 22.
显示于类别:	[牙醫學系暨碩士班] 期刊論文

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