中山醫學大學機構典藏 CSMUIR:Item 310902500/11305
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    题名: Hypoxia inducible factor-1α expression in areca quid chewing-associated oral squamous cell carcinomas.
    作者: Lee, SS
    Tsai, CH
    Yang, SF
    Ho, YC
    Chang, YC
    贡献者: 中山醫學大學
    日期: 2010
    上传时间: 2015-07-15T09:03:54Z (UTC)
    摘要: OBJECTIVES:
    Hypoxia inducible factor (HIF)-1α gene expression is mainly induced by tissue hypoxia. Overexpression of HIF-1α has been demonstrated in a variety of cancers. The aim of this study was to compare HIF-1α expression in normal human oral epithelium and areca quid chewing-associated oral squamous cell carcinoma (OSCC) and further to explore the potential mechanisms that may lead to induce HIF-1α expression.
    METHODS:
    Twenty-five OSCC from areca quid chewing-associated OSCC and 10 normal oral tissue biopsy samples without areca quid chewing were analyzed by immunohistochemistry. The oral epithelial cell line GNM cells were challenged with arecoline, a major areca nut alkaloid, by using Western blot analysis. Furthermore, glutathione precursor N-acetyl-l-cysteine (NAC), AP-1 inhibitor curcumin, extracellular signal-regulated protein kinase inhibitor PD98059, and protein kinase C inhibitor staurosporine were added to find the possible regulatory mechanisms.
    RESULTS:
    Hypoxia inducible factor-1α expression was significantly higher in OSCC specimens than normal specimen (P<0.05). Arecoline was found to elevate HIF-1α expression in a dose- and time-dependent manner (P<0.05). The addition of NAC, curcumin, PD98059, and staurosporine markedly inhibited the arecoline-induced HIF-1α expression (P<0.05).
    CONCLUSIONS:
    Hypoxia inducible factor-1α expression is significantly upregulated in areca quid chewing-associated OSCC and HIF-1α expression induced by arecoline is downregulated by NAC, curcumin, PD98059, and staurosporine.
    © 2010 John Wiley & Sons A/S.
    URI: https://ir.csmu.edu.tw:8080/ir/handle/310902500/11305
    http://dx.doi.org/10.1111/j.1601-0825.2010.01680.x
    關聯: Oral Dis. 2010 Oct;16(7):696-701.
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