Hypoxia inducible factor-1α expression in areca quid chewing-associated oral squamous cell carcinomas.

doi:10.1111/j.1601-0825.2010.01680.x

中山醫學大學機構典藏 CSMUIR > 口腔醫學院 > 牙醫學系暨碩士班 > 期刊論文 > Item 310902500/11305

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/11305

Title:	Hypoxia inducible factor-1α expression in areca quid chewing-associated oral squamous cell carcinomas.
Authors:	Lee, SS Tsai, CH Yang, SF Ho, YC Chang, YC
Contributors:	中山醫學大學
Date:	2010
Issue Date:	2015-07-15T09:03:54Z (UTC)
Abstract:	OBJECTIVES: Hypoxia inducible factor (HIF)-1α gene expression is mainly induced by tissue hypoxia. Overexpression of HIF-1α has been demonstrated in a variety of cancers. The aim of this study was to compare HIF-1α expression in normal human oral epithelium and areca quid chewing-associated oral squamous cell carcinoma (OSCC) and further to explore the potential mechanisms that may lead to induce HIF-1α expression. METHODS: Twenty-five OSCC from areca quid chewing-associated OSCC and 10 normal oral tissue biopsy samples without areca quid chewing were analyzed by immunohistochemistry. The oral epithelial cell line GNM cells were challenged with arecoline, a major areca nut alkaloid, by using Western blot analysis. Furthermore, glutathione precursor N-acetyl-l-cysteine (NAC), AP-1 inhibitor curcumin, extracellular signal-regulated protein kinase inhibitor PD98059, and protein kinase C inhibitor staurosporine were added to find the possible regulatory mechanisms. RESULTS: Hypoxia inducible factor-1α expression was significantly higher in OSCC specimens than normal specimen (P<0.05). Arecoline was found to elevate HIF-1α expression in a dose- and time-dependent manner (P<0.05). The addition of NAC, curcumin, PD98059, and staurosporine markedly inhibited the arecoline-induced HIF-1α expression (P<0.05). CONCLUSIONS: Hypoxia inducible factor-1α expression is significantly upregulated in areca quid chewing-associated OSCC and HIF-1α expression induced by arecoline is downregulated by NAC, curcumin, PD98059, and staurosporine. © 2010 John Wiley & Sons A/S.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/11305 http://dx.doi.org/10.1111/j.1601-0825.2010.01680.x
Relation:	Oral Dis. 2010 Oct;16(7):696-701.
Appears in Collections:	[牙醫學系暨碩士班] 期刊論文

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