Apoptotic and anti-proliferative effects of 17β-estradiol and 17β-estradiol-like compounds in the Hep3B cell line

doi:10.1007/s11010-005-9000-y

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题名:	Apoptotic and anti-proliferative effects of 17β-estradiol and 17β-estradiol-like compounds in the Hep3B cell line
作者:	Huang, Erh-Jung Wu, Cheng-Chung Huang, Hsien-Ping Liu, Jer-Yuh Lin, Chung-Sheng Chang, Yan-Zin James, A.Lin Lin, Jaung-Geng Chen, Li-Mien Lee, Shin-Da Kuo, Wei-Wen Huang, Chih-Yang
贡献者:	中山醫學大學
关键词:	hepatocellular carcinoma (HCC);17 β-estrodiol;17 β-estrodiol-like compounds;apoptotic and antiproliferative effects
日期:	2006
上传时间:	2015-07-15T04:39:11Z (UTC)
ISSN:	0300-8177
摘要:	Since there is evidence for estrogen and estrogen-like compounds to have beneficial effect on the pathogenesis of hepatocellular carcinoma (HCC), this study was designed to investigative the apoptotic and anti-proliferative effects of these compounds on the human hepatoma Hep3B cell line. The Hep3B cells were treated with 17β-estradiol (E2), diethylstilbestrol (DES), tamoxifen, and genistein. After treatments of these compounds at the concentration of 10-6 or 10-8 M, the Hep3B cells were demonstrated to have significant DNA fragmentation, nucleus condensation, cytochrome-c leaking from the mitochondria and caspase-3 activation by DAPI and Western blotting. The cells were also observed to have declined proliferative potential by MTT assay, arrested cell cycle by flow-cytometry measurements. However, the cytochrome-c leaking from the mitochondria induced by E2 and E2-like compounds was blocked totally by ICI 182,780 treatment. These finding suggest that estrogen and the estrogen-like compounds may induce anti-proliferative and apoptotic effects in Hep3B cells, and the E2 and the E2-like compounds mediated apoptotic effect was estrogen receptor dependent. Among the drugs tested, E2, E2 agonists (DES and genistein) and partial antagonist (tamoxifen), all showed the stronger anti-tumor potential.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/11262 http://dx.doi.org/10.1007/s11010-005-9000-y
關聯:	Molecular and Cellular Biochemistry October 2006, Volume 290, Issue 1-2, pp 1-7
显示于类别:	[醫學系] 期刊論文

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