Sonic hedgehog promotes porcine oocyte maturation and early embryo development.

doi:10.1071/RD08277

中山醫學大學機構典藏 CSMUIR > 健康管理學院 > 營養學系暨碩士班 > 期刊論文 > Item 310902500/11240

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/11240

Title:	Sonic hedgehog promotes porcine oocyte maturation and early embryo development.
Authors:	Nguyen, NT Lin, DP Yen, SY Tseng, JK Chuang, JF Chen, BY Lin, TA Chang, HH Ju, JC
Contributors:	中山醫學大學
Date:	2009
Issue Date:	2015-07-14T08:23:32Z (UTC)
Abstract:	In the present study, we investigated the effects of the Sonic hedgehog (Shh) protein on porcine oocyte maturation and early embryo development. Immunohistochemistry showed activation of Shh signalling in cumulus-oocyte complexes (COCs), as reflected by Patched (Ptc), Smoothened (Smo) and Gli1 expression in oocytes, cumulus cells and granulosa cells, particularly those of small follicles (<2 mm in diameter). Western blot analysis showed Smo expression in COCs and in denuded oocytes derived from small and medium (3-7 mm)-sized follicles. Small follicles contained the highest concentration of Shh in follicular fluid compared with medium-sized and large (>7 mm in diameter) follicles. Supplementation with Shh (0.5 or 1 microg mL(-1)) enhanced oocyte maturation compared with the control group (92.4% and 90.4% v. 81.9%, respectively; P < 0.05). This effect was reversed by the simultaneous addition of cyclopamine (1-2 microm), an Shh inhibitor. Similar to intact COCs, denuded COCs showed enhanced maturation following Shh supplementation. Furthermore, cyclin B1 content, extracellular signal-regulated kinase 1/2 phosphorylation, intracellular calcium release, blastocyst rate and total cell numbers were greater (P < 0.05) in oocytes matured in the presence of 0.5 and 1 microg mL(-1) Shh compared with control oocytes. The findings of the present study provide the first evidence that the Shh signalling pathway is active, or at least partially activated, in the porcine ovary and is likely to promote oocyte cytoplasmic and nuclear maturation, as well as subsequent in vitro development, although the underlying mechanisms remain to be elucidated. PMID: 19567223 [PubMed - indexed for MEDLINE]
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/11240 http://dx.doi.org/10.1071/RD08277
Relation:	Reprod Fertil Dev. 2009;21(6):805-15.
Appears in Collections:	[營養學系暨碩士班] 期刊論文

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