Odontogenic differentiation of human dental pulp cells by calcium silicate materials stimulating via FGFR/ERK signaling pathway.

doi:10.1016/j.biomaterials.2011.09.007

中山醫學大學機構典藏 CSMUIR > 口腔醫學院 > 牙醫學系暨碩士班 > 期刊論文 > Item 310902500/11120

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題名:	Odontogenic differentiation of human dental pulp cells by calcium silicate materials stimulating via FGFR/ERK signaling pathway.
作者:	Liu, CH CJ, Hung TH, Huang CC, Lin CT, Kao Shie, MY
貢獻者:	中山醫學大學
日期:	2014
上傳時間:	2015-07-10T05:34:49Z (UTC)
ISSN:	0142-9612
摘要:	Bone healing needs a complex interaction of growth factors that establishes an environment for efficient bone formation. We examine how calcium silicate (CS) and tricalcium phosphate (β-TCP) cements influence the behavior of human dental pulp cells (hDPCs) through fibroblast growth factor receptor (FGFR) and active MAPK pathways, in particular ERK. The hDPCs are cultured with β-TCP and CS, after which the cells' viability and odontogenic differentiation markers are determined by using PrestoBlue® assay and western blot, respectively. The effect of small interfering RNA (siRNA) transfection targeting FGFR was also evaluated. The results showed that CS promoted cell proliferation and enhances FGFR expression. It was also found that CS increases ERK and p38 activity in hDPCs, and furthermore, raises the expression and secretion of DSP, and DMP-1. Additionally, statistically significant differences (p<0.05) have been found in the calcium deposition in si-FGFR transfection and ERK inhibitor between CS and β-TCP; these variations indicated that ERK/MAPK signaling is involved in the silicon-induced odontogenic differentiation of hDPCs. The current study shows that CS substrates play a key role in odontoblastic differentiation of hDPCs through FGFR and modulate ERK/MAPK activation.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/11120 http://dx.doi.org/10.1016/j.biomaterials.2011.09.007
關聯:	Mater Sci Eng C Mater Biol Appl. 2014 Oct;43:359-66.
顯示於類別:	[牙醫學系暨碩士班] 期刊論文

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