Genetic and cellular characterizations of human TCF4 with microsatellite instability in colon cancer and leukemia cell lines.

doi:10.1016/j.canlet.2005.03.011

中山醫學大學機構典藏 CSMUIR > 醫學院 > 生化微生物免疫研究所 > 期刊論文 > Item 310902500/11023

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题名:	Genetic and cellular characterizations of human TCF4 with microsatellite instability in colon cancer and leukemia cell lines.
作者:	Chang, HR Cheng, TL Liu, TZ Hu, HS Hsu, LS Tseng, WC Chen, CH Tsao, DA.
贡献者:	中山醫學大學
关键词:	10.1016/S0378-4274(96)03801-5
日期:	2006
上传时间:	2015-07-02T10:38:03Z (UTC)
ISSN:	0304-3835
摘要:	It has been reported that the mutational inactivation of the adenomatous polyposis coli (APC) and beta-catenin genes play important roles in colorectal carcinogenesis. However, alteration of the components in the Wnt signaling pathway in colorectal cancer (CRC) with microsatellite instability (MSI) has been elucidated. To define the precise role of the Wnt signaling components in CRC and leukemia cell lines with MSI, mutational analyses of the T cell factor 4 (TCF4) genes were performed. Here we describe for the first time a TCF4 MSI+ phenotype in leukemia cell lines except in colon cancer cell lines. Moreover, we found that these cell lines exhibited deletion and insertion of 1-2A in an (A)9 repeat so as to result in (A)7, (A)8, (A)10 and (A)11 repeat, respectively. To characterize the cellular function of these special TCF4 mutant clones, transient transfection and fluorescent microscopy were analyzed and the results revealed that the TCF4 frameshift gene products all localized in nuclei. Surprisingly, these TCF4 frameshift mutants lost transcriptional activity with beta-catenin and down-regulate the target gene expression. These results delineate a novel role for MSI+TCF4 in leukemia and colon cancer progression.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/11023 http://dx.doi.org/10.1016/j.canlet.2005.03.011
關聯:	Cancer Lett. 2006 Feb 20;233(1):165-71.
显示于类别:	[生化微生物免疫研究所] 期刊論文

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