|
English
|
正體中文
|
简体中文
|
Items with full text/Total items : 17938/22957 (78%)
Visitors : 7399543
Online Users : 235
|
|
|
Loading...
|
Please use this identifier to cite or link to this item:
https://ir.csmu.edu.tw:8080/ir/handle/310902500/10759
|
Title: | The suppressive effect of Rho kinase inhibitor, Y-27632, on oncogenic Ras/RhoA induced invasion/migration of human bladder cancer TSGH cells. |
Authors: | Chang, HR Huang, HP Kao, YL Chen, SL Wu, SW Hung, TW Lian, JD Wang, CJ |
Contributors: | 中山醫學大學 |
Keywords: | Bladder cancer;Ras;RhoA;RhoA kinase inhibitor |
Date: | 2010 |
Issue Date: | 2015-05-08T08:09:36Z (UTC)
|
ISSN: | 0009-2797 |
Abstract: | Urothelial cell carcinoma is the most common type of malignancy found in long-term dialysis patients and kidney transplant recipients in Taiwan. Surgical specimens of tumorous and non-tumorous bladder tissues were collected from 12 patients with bladder cancer. Increased expressions of Ras, RhoA, Akt, PI-3K were demonstrated in the tumors as compared to adjacent control tissues. To understand the impact of Ras over-expression on bladder cancer progression, human bladder cancer TSGH 8301 cells were transfected with Ras DNA. The Ras-transfected cells were then treated with either a PI-3K inhibitor (wortmannin) or Rho kinase inhibitor (Y-27632) and the expressions of Ras, PI-3K, Akt, NF-kappaB, and RhoA were analyzed. Fluorescent phalloidin staining demonstrated more intense F-actin staining in the Ras over-expressed cells than in the control cells, and the intensity of F-actin was inhibited by Y-27632. A gelatin zymography study demonstrated that the MMP-2 and MMP-9 expressions of the Ras-transfected cells were enhanced, and Y-27632 treatment reduced the levels of MMP-2 and MMP-9. Similarly, a wound healing assay revealed that the ability of cell migration was markedly increased by Ras transfection and the healing rate after treatment of Y-27632 was delayed. Our results provide evidence that Ras-induced RhoA and NF-kappaB activation was involved in the invasion/migration of bladder cancer. Through Ras and/or RhoA inhibition, there might be an opportunity for new therapeutic interventions in bladder cancer. |
URI: | https://ir.csmu.edu.tw:8080/ir/handle/310902500/10759 http://dx.doi.org/10.1016/j.cbi.2009.10.018 |
Relation: | Chem Biol Interact. 2010 Jan 5;183(1):172-80. |
Appears in Collections: | [生化微生物免疫研究所] 期刊論文
|
Files in This Item:
File |
Description |
Size | Format | |
index.html | 期刊論文 | 0Kb | HTML | 386 | View/Open |
|
All items in CSMUIR are protected by copyright, with all rights reserved.
|