Mutation of p53 gene codon 63 in saliva as a molecular marker for oral squamous cell carcinomas.

doi:10.1016/S1368-8375(00)00005-1

中山醫學大學機構典藏 CSMUIR > 口腔醫學院 > 牙醫學系暨碩士班 > 期刊論文 > Item 310902500/10648

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题名:	Mutation of p53 gene codon 63 in saliva as a molecular marker for oral squamous cell carcinomas.
作者:	Liao, PH Chang, YC Huang, MF Tai, KW Chou, MY
贡献者:	中山醫學大學
日期:	2000
上传时间:	2015-04-13T09:37:07Z (UTC)
ISSN:	1368-8375
摘要:	The inactivation of tumor suppressor gene (TSG) is important during multistage carcinogenesis. The p53 TSG is frequently mutated in oral squamous cell carcinomas. These mutations can serve as very specific markers for the presence of tumor cells in a background of normal cells. In this study, 10 oral squamous cell carcinoma patients and 27 normal dental students were collected from Chung Shan Medical and Dental College Hospital, Taichung, Taiwan. Extractions of DNA from saliva were obtained. Exon 4 and intron 6 within the p53 gene were amplified with polymerase chain reactions (PCRs) followed by DNA sequence analysis. DNA sequence analysis of PCR products revealed that five of eight (62.5%) tumor saliva samples and five of 27 (18. 52%) healthy saliva samples contained p53 exon 4 codon 63 mutations. These results were significantly different by using Chi-square test (P<0.05). The majority of the base substitutions were C deletions. Probable hot spots for the mutation were identified at exon 4 codon 63, which has not been observed before in head and neck cancers. Our study indicated that mutation of p53 codon 63 in saliva might be a molecular marker for oral squamous cell carcinomas. In addition, the amount of DNA recovered from saliva in most cases is sufficiently large and its quality suitable to enable PCR amplification which could be used in the search for mutations. The protocol described is rapid, cheap, and easy to perform, and may be useful for epidemiological studies for oral carcinogenesis.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/10648 http://dx.doi.org/10.1016/S1368-8375(00)00005-1
關聯:	Oral Oncol. 2000 May;36(3):272-6.
显示于类别:	[牙醫學系暨碩士班] 期刊論文

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