Matrix metalloproteinase 2 and matrix metalloproteinase 9 expression in human oral squamous cell carcinoma and the effect of protein kinase C inhibitors: preliminary observations.

中山醫學大學機構典藏 CSMUIR > 口腔醫學院 > 牙醫學系暨碩士班 > 期刊論文 > Item 310902500/10615

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://ir.csmu.edu.tw:8080/ir/handle/310902500/10615

题名:	Matrix metalloproteinase 2 and matrix metalloproteinase 9 expression in human oral squamous cell carcinoma and the effect of protein kinase C inhibitors: preliminary observations.
作者:	Tsai, CH Hsieh, YS Yang, SF Chou, MY Chang, YC
贡献者:	中山醫學大學
日期:	2003
上传时间:	2015-04-10T07:47:46Z (UTC)
摘要:	OBJECTIVE: The purpose of this study was to investigate gelatinase (matrixmetalloproteinase [MMP]-2 and MMP-9) expression in oral squamous cell carcinoma (OSCC) and to explore the mechanisms that may inhibit gelatinase activity. STUDY DESIGN: Thirty biopsy specimens of OSCCs were examined by means of immunohistochemistry. Supernatants from primary cultures of human oral mucosal keratinocyte and oral cancer-derived cells (KB and OC2) were analyzed by means of gelatin zymography. Furthermore, protein kinase C (PKC) inhibitors (H7 and staurosporine) were added to test how they modulate gelatinase production in human oral cancer cells. RESULTS: MMP-2 and MMP-9 expression was significantly higher in oral SCCs and was located in discrete clusters of tumor cells. Oral mucosal keratinocyte cultures, KB, and OC2 were found to secrete and produce MMP-2 and MMP-9. However, the amounts of MMP-2 and MMP-9 were highly elevated in the 2 oral cancer cell lines in comparison with oral mucosal keratinocyte cultures (P <.05). In addition, PKC inhibitors were found to decrease MMP-2 and MMP-9 activities in oral cancer cells (P <.05). CONCLUSION: Taken together, human oral SCCs produce MMP-2 and MMP-9 in vivo and in vitro, and gelatinase activity is down-regulated by PKC inhibitors in vitro. PKC inhibitors suppressing MMP production and/or activity may represent valuable therapeutic agents through their influence on the pathogenesis of OSCC. These agents may prove clinically useful in combination with standard therapeutic modalities for the treatment of patients with oral cancer.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/10615
關聯:	Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003 Jun;95(6):710-6.
显示于类别:	[牙醫學系暨碩士班] 期刊論文

文件中的档案:

档案	描述	大小	格式	浏览次数
index.html	報導	0Kb	HTML	472	检视/开启

检视Licence

在CSMUIR中所有的数据项都受到原著作权保护.

TAIR相关文章

数据加载中.....