蛋白激C及γ-麩胺胜在感染中華肝吸蟲大白鼠體內之活化表現

中山醫學大學機構典藏 CSMUIR > 醫學院 > 生化微生物免疫研究所 > 博碩士論文 > Item 310902500/943

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/943

Title:	蛋白激C及γ-麩胺胜在感染中華肝吸蟲大白鼠體內之活化表現 γ-GTase Foci Development and PKC Activation in the Clonorchis Sinensis Infected Rats
Authors:	蘭光坪 Lan, Kuang-Pin
Contributors:	中山醫學院：生化科學研究所;李秀雄;蕭松瑞
Keywords:	中華肝吸蟲;蛋白激C
Date:	1997
Issue Date:	2010-03-25T03:37:29Z (UTC)
Abstract:	為探討中華肝吸蟲的致病機轉，本研究以wistar 雄性大白鼠為實驗動物，研究分實驗組及正常組，實驗組以口餵管餵食中華肝吸蟲囊蚴後再公為投藥組及不投藥組，投藥組於感染後十二週以Praziquantel處理，不投藥組僅給予生理食鹽水。三組於感染後十三週均以斷頭方法，分別取其血液、糞便及肝膽組織以傳統的方法評估，另將肝膽組織萃取液以組織生化電泳分析（Sodium dodecyl sulfate ployacrylamide, SDS-PAGE）與西方墨點法（Western blot）分析該組織是否有特異性蛋白出現及偵測其Protein kinase C(PKC)及原致癌基因c-Jun、c-Fos 蛋白表現量，此外，藉冷凍組織切片分析（γ-glutamyl transpeptidase, γ-GTase）γ-GTase活性的表現，來探討Praziquantel 對中華肝吸蟲感染大白鼠之作用機轉。　　實驗結果，較之不投藥組，投藥組之γ－GTase foci的面積明顯減少，且投藥組與不投藥組之間的PKC有顯著差異，而原致癌基因c-Jun、c-Fos 實驗組與正常組則均無差異表現。至於傳統的實驗評估僅組織病理切片染色有有顯著的差異；投藥組之大白鼠膽管沒有成蟲的寄生，而不投藥組則可以發現成蟲。其餘之病理及血液常規、生化，糞便蟲卵等僅些微差異。　　根據以上的實驗結果，我們初步推測中華肝吸蟲的致病機轉可能和 PKC 的活化作用、γ-GTase 活性的表現有關，而驅蟲藥 Praziquantel 治療中華肝吸蟲，除了直接破壞蟲體外，也可能會抑制 PKC 的表現進而調節γ-GTase 的活性，藉以提升細胞的免疫修復，而降低癌化的產生；唯其詳細機轉有待進一步探討。　　The purpose of this study was to investigate the pathogenic mechanism of Clonorchis sinensis. Male wistar rats were utilized as experimental animals and randomly divided into three groups. Two of them, which were divided into drug-treated and control groups were fed with metacercaria of Clonorchis sinensis. The drug-treated rats were treated with Praziquantel after 12 weeks of infection, the control rats were treated with saline.13 weeks after infection,all subjects were sacrificed to collect, serum, stool liver andgall-bladder tissues which were further analyzed by traditional methods. The extracts of liver and gall-bladder tissues were analyzed with Sodium dodecyl sulfate ployacrylamide (SDS-PAGE)and western blot to identify specific proteins including protein kinase C(PKC) and the products of the protooncogenes such as c-Jun andd c-Fos. The pathogenic mechanism was also dissected from analysis of the γ-GTase activity in frozen sections. 　　The results showed that the area of γ-GTase foci of drug-treated group was significantly reduced as compared to that of control group. Furthermore, there is a significant difference in PKC between drug-treated and non-treated groups. However, no significant difference was found in the level of c-Jun and c-Fos. In traditional experiment, H-E stain didn''t showed the presence of adult worm in bile ducts of drug-treated rats, while they could be detected in control rats. The other pathogenic parameters, such as blood routine and stool egg only showed mild difference. 　　According to the eresults described above, we preliminarily suggested that the pathogenic mechanism of Clonorchis sinensis may be related to activation of PKC and increased expression of γ-GTase.Praziquantel, not only can destroy adult worm directly, but also regulate γ-GTase activity. Furthermore, by inhibiting the expression of PKC, Praziquantel can elevate the repair of cells and reduce tumorigenesis. Further investigations are needed to dissect the detailed mechanism.
URI:	http://140.128.138.153:8080/handle/310902500/943
Appears in Collections:	[生化微生物免疫研究所] 博碩士論文

Files in This Item:

There are no files associated with this item.

Loading...