嘉寶果水萃物改善糖尿病腎病變(第二型)之研究

中山醫學大學機構典藏 CSMUIR > 醫學院 > 生化微生物免疫研究所 > 博碩士論文 > Item 310902500/9009

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/9009

Title:	嘉寶果水萃物改善糖尿病腎病變(第二型)之研究 Myrciaria cauliflora extracts attenuate diabetic nephropathy in type II DM mice.
Authors:	施懿宸 Shih, Yi-Chen
Contributors:	中山醫學大學：生化暨生物科技研究所;黃惠珮
Keywords:	嘉寶果萃取物;鏈佐黴素;菸鹼胺;糖尿病腎病變 Myrciaria cauliflora extracts (MCE);Streptozotocin (STZ);Nicotinamide (NA);Diabetic nephropathy (DN)
Date:	2013
Issue Date:	2014-01-15T04:43:56Z (UTC)
Abstract:	在全世界中糖尿病腎病變為主要導致末期腎臟病的原因之一，且致死率於近年來不斷地攀升。過去的研究指出高血糖誘導的腎臟受損，活性氧物種(自由基)扮演一個重要的角色。嘉寶果，俗稱樹葡萄，目前多被研究是否能成為機能性食品，因富含花青素具有抗氧化的及抗發炎的特性。本研究旨在探討嘉寶果萃取物(MCE)是否能減緩第二型糖尿病小鼠的腎病變發展。首先，將 MCE 藉由分光光度計及高液相層析儀分析花青素及多酚的組成。在動物實驗方面，將 C57BL/6J 小黑鼠以 100 毫克/公斤之鏈佐黴素(streptozotocin, STZ)與 240 毫克/公斤之菸鹼胺(Nicotinamide, NA)注射腹腔並餵予高脂飼料誘導糖尿病及給予各種濃度的 MCE(0.1,0.5,1 %)。於每兩個禮拜監測一次血糖濃度、體重、葡萄糖耐受性測試，胰島素耐受性測試、血壓、腎臟超音波；犧牲後做腎臟切片分析 H&E 染色、PAS 染色、MASSON 式染色及免疫組織化學染色(IHC)。結果顯示，MCE 能穩定第二型糖尿病小鼠的血糖代謝並間接證實能改善胰島素敏感性，除此之外，糖尿病腎病變導致的腎臟重量減輕、腎絲球萎縮、醣類堆積及膠原蛋白增生等現象，在 MCE 的給予之下也有所改善。在細胞實驗部分，利用高糖誘導 MES 13 腎絲球間質細胞增生，再觀察 MCE 對其抑制作用與相關機制。結果指出 MCE 可透過調節 Ras/PI3K/Akt 路徑而抑制腎絲球腎間質細胞增生，同時可減少ollagen IV 及 Fibronectin 等纖維化相關因子。本實驗證實 MCE 能延緩與改善糖尿病腎病變的病程，未來期待可進一步發展為一保健食品。 Diabetic nephropathy (DN) is a major cause of end-stage renal disease (ESRD) and the mortality is continuously progressing worldwide. Previous studies indicate that reactive oxygen species play an important role in high glucose-induced renal injury. Myrciaria cauliflora has been reported as a functional food rich in anthocyanins possessing anti-oxidative and anti-inflammative properties. This study examined whether Myrciaria cauliflora extracts (MCE) can attenuate diabetic nephropathy progression in type 2 DM mice. First, the composition of anthocyanins and polyphenols of MCE were determined by HLPC and spectrophotometer. One hundred mg/kg of streptozotocin and two hundred forty mg/kg nicotinamide were administered to C57BL/6J mice fed with high fat diet and variant concentrations of MCE. The plasma glucose concentration, body weight, oral glucose tolerance, insulin tolerance, blood pressure, renal ultrasoundultrasonic wavewere monitored every other two weeks. After sacrifice, the kidneysof mice were analyzed by using H&E, PAS, MASSON and IHC staining.The results showed that MCE stabilized the plasma glucose and indirect improved the insulin sensitivity in type 2 DM mice. In addition, diabetes-caused glomerular atrophy, accumulation of saccharide, andproliferation of collagen IVwere recovered or reduced under treatment with MCE in diabetic mice. Our results indicate that MCE has beneficial effects in diabetic nephropathy and the mechanism has been confirmed in vitro which inhibit proliferation via Ras/PI3K/Aktand kidney fibrosis related proteins.Comprehensive, this work will provide the potentialfor MCE asa natural food inhibiting the diabetic nephropathy.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/9009
Appears in Collections:	[生化微生物免疫研究所] 博碩士論文

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