We investigate the feasibility of coupling the quartz crystal microbalance (QCM) with magnetic separation for on-line analysis. The deposited matrix was serviced as selective binder to target species pulsed injection into the flow stream. Frequency counter was used to monitor the deposition and immunoreactions of nanoparticles.
The QCM gives a response that is characteristic of species deposited on the electrode surface. The deposition of magnetic beads on the electrode surface is simpler and less mechanical stress to biomolecules than that of chemical immobilized biomolecules on the electrode surface. This technique is also easy to automation and is useful for detecting biomarkers.
The results show several different in clip-tied flow cell and screw-tied flow cell. In regarding to the responsesof magnetic nanoparticles, the frequency shift from clip-tied cell is slightly less than that from screw-tied cell , but its reproducibility is better. On the other hand , screw-tied flow cell in viscosity test is more fitting in with real state. The clip-tied flow cell is quick to assemble and to reach the steady state of the frequency response of the QCM. The results provide us several reference material to select a suitable system.
