| 摘要: | 以宿主肺臟組織蛋白變化之現象, 評估不同之γ-aminobutyricacid(
GABA)類驅蟲藥VD-99-11及Milbemycin D, 治療感染廣東住血線蟲第三期
幼蟲的大白鼠之治療效果, 作為與傳統治療效果之比較, 以探討其優劣性
。實驗結果在感染廣東住血線蟲而未治療及以VD-99-11治療組之老鼠肺臟
組隻微粒體的SDS-PAGE電泳分析中發現分子量22-KDa 有一明顯蛋白質,
然未感染及以Milbemycin D治療組之老鼠肺臟組織的微粒體卻沒有相同蛋
白質; 使用Superdex 200 column 作連續層析法純化, 鑑定此蛋白質其化
學性及免疫性為鐵蛋白(ferritin), 再利用等電點電泳法及離子交換層析
法確認其為L ferritin。觀察應用免疫組織染色法之病理切片, 在感染廣
東住血線蟲肺臟組織有發炎現象, 浸潤之吞噬細胞內也有ferritin之堆積
。因此, 推測鐵蛋白(ferritin)為感染廣東住血線蟲致病機轉之重要因素
。據此, 我們認為以生化方法評估治療效果方法正確同時也可作為探討致
病機轉之研究方法之一。
Protein variation of the lung tissue in the rats, which
infected for Angiostrongylus cantonensis third larvae were
traeted with VD-99-11 and milbemycin D, compared with the
effects of traditional chemotherapeutics. Sodium dodecyl
sulfate-polyacrylamide gel electrophoresis techniques wereused
to analyze the biochemistry differences of the lung tissue. We
found the 22-KDa protein was quantitatively increaseds in the
lung microsomes from the Angiostrongyluscantonensis infected and
incured rats, however, it was almost absent in normal and cured
rats. The protein waspurified by sequential chromatography on
Superdex 200 column, and was identified chemically and
immunologically as ferritin. Moreover, using isoelectric
focusing and anion exchange chromatography, it was further known
as L ferritin. The distribution of this 22-KDa protein in the
lung tissueof the Angiostrongylus cantonensis infected rats was
studies by immunocytochemistry. The positively stained cells
weremainly infiltration macrophages. |
