| Abstract: | 目前傳統癌症治療方法分為化學治療及放射線治療;化學治療被稱為最有效的癌症治療方式，是利用一種或多種的藥物來合併使用(又稱為合併性化學治療)，而其作用是在破壞癌細胞生長，達到治療癌症、控制病情、緩解症狀的目的。但因化學治療除了殺死癌細胞外，也會對正常生長較快速的細胞造成傷害而產生副作用。而放射線治療即是運用較高能量的放射線，約是診斷用的X光之能量的數百或數千倍，來破壞癌細胞，防止癌細胞的生長與分裂，也是目前治療肺癌的主要策略之一。但對於正常組織也是有劑量耐受性的限制，而放射線對於不同癌細胞也有不同的感受性，且放射線對於癌症病人也具有相當程度的副作用；本研究之目的為利用berberine黃連素此中草藥來合併放射線治療，以期能輔助對肺癌細胞及腫瘤的毒殺性，甚至可以使放射線的劑量可以降低，以降低癌症病人的副作用。在動物實驗中，?用裸鼠在皮下種植人類肺癌細胞A549，發現當?鼠給予berberine 合併放射線時會明顯地抑制腫瘤大小，再以Western blot方法分析蛋白表現，探討其作用的機轉，由實驗結果Bcl-2、caspase-3、ATG-5及LC-3的蛋白表現量顯示，使用黃連素合併放射線實驗中在apotosis及autophage並無顯著的相關性。
The current conventional cancer treatments are divided into chemotherapy and radiation treatment; chemical treatment known as the most effective cancer treatment the use of one or more of the drugs in combination (also known as combined chemotherapy), and its role in destruction of cancer cells, to cure cancer, control the disease, relieve symptoms purposes. Addition of chemotherapy to kill cancer cells, but also for normal cell growth and more rapid damage and side effects. The use of radiation therapy that is high energy radiation, is about the diagnostic X-ray energy of hundreds or thousands of times, to destroy cancer cells, prevent cancer cell growth and division, is currently the main strategy of the treatment of lung cancer one. But also a dose of normal tissue tolerance limits, and radiation for different cells have different sensitivity, and radiation for cancer patients also have a considerable degree of side effects; Purpose of this study is the use of this herbal medicine berberine to radiation therapy combined with a view to assist in lung cancer cells and tumor cytotoxic, or even to reduce the radiation dose to reduce side effects of cancer patients. In animal experiments, using nude mice in the subcutaneous growing human lung cancer cells A549, found that aging rats given berberine combined radiation will significantly inhibit tumor size, and then to Western blot to analyze protein expression, to explore its role in the mechanism of, the experimental results Bcl-2, caspase-3, ATG-5 and LC-3 protein expression level display, use of berberine combined radiation experiments in apotosis and autophage, no significant correlation. |
