Dangshen (Codonopsis pilosula) Activates IGF-I and FGF-2 Pathways to Induce Proliferation and Migration Effects in RSC96 Schwann Cells

doi:10.1142/S0192415X10007907

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Title:	Dangshen (Codonopsis pilosula) Activates IGF-I and FGF-2 Pathways to Induce Proliferation and Migration Effects in RSC96 Schwann Cells
Authors:	Chen, Hsien-Tung Tsai, Ying-Lan Chen, Yueh-Sheng Jong, Guo-Ping Chen, Wei-Kung Wang, Hwai-Lee Tsai, Fuu-Jen Tsai, Chang-Hai Lai, Tung-Yuan Tzang, Bor-Show Huang, Chih-Yang Lu, Chung-Yen
Contributors:	中山醫學大學醫學系生化科
Keywords:	Dangshen Proliferate and Migrate Effect Schwann Cells Peripheral Nerve Regeneration
Date:	2010
Issue Date:	2012-08-10T08:03:16Z (UTC)
ISSN:	0192-415X
Abstract:	This study evaluates the proliferative and migrative effects of dangshen on RSC96, Schwann cells. We investigated the molecular signaling pathways, which include: (1) survival signaling, IGFs-IGFIR-Akt-Bcl2 and proliferative signaling, cell cycle factors and MAPK pathways. (2) migrate and anti-scar signaling, FGF-2-uPA-MMPs. After treatment with different concentrations (20 μg/ml, 40 μg/ml, 60 μg/ml, 80 μg/ml, and 100 μg/ml) of dangshen. We observed a dose dependent proliferative effect using PCNA Western blotting assay, MTT assay and the wound healing test. We also found that dangshen stimulates the protein expressions of IGF-I pathway regulators, cell cycle controlling proteins and excites the MAPK signaling pathway regulators ERK and P38. Dangshen even stimulates the FGF-2-uPA-MMP 9 migration pathway in RSC 96 Schwann cells. Using MAPK chemical inhibitors, U0126, SB203580, and SP600125, the proliferative effects of dangshen on RSC 96 cells were identified to be ERK- and P38- dependent. Based on these results, applying an appropriate dose of dangshen with biomedical materials would be a potential approach for enhancing neuron regeneration. Read More: http://www.worldscientific.com/doi/ref/10.1142/S0192415X10007907
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/4443 http://dx.doi.org/10.1142/S0192415X10007907
Relation:	Am. J. Chin. Med 38, 359 (2010)
Appears in Collections:	[醫學系] 期刊論文

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