Spinal glutamatergic NMDA-dependent pelvic nerve-to-external urethra sphincter reflex potentiation caused by a mechanical stimulation in anesthetized rats.

doi:10.1152/ajprenal.00443.2006

中山醫學大學機構典藏 CSMUIR > 醫學院 > 醫學系 > 期刊論文 > Item 310902500/3613

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题名:	Spinal glutamatergic NMDA-dependent pelvic nerve-to-external urethra sphincter reflex potentiation caused by a mechanical stimulation in anesthetized rats.
作者:	Jiuan-Miaw Liao;Pei-Chen Huang;Shwu-Fen Pan;Mei-Jung Chen;Kwong-Chung Tung;Hsien-Yu Peng;Jyh-Cherng Shyu;Ying-Ming Liou;Gin-Den Chen;Tzer-Bin Lin
贡献者:	中山醫學大學:醫學系
关键词:	pelvic nerve;urethra;LTP;AMPA
日期:	2007
上传时间:	2011-03-14T03:51:47Z (UTC)
摘要:	The current study investigates whether the spinal pelvic nerve-to-external urethra sphincter (EUS) reflex potentiation can be induced by a mechanical stimulation and whether the glutamatergic mechanism is involved in yielding such a reflex potentiation. The external urethra sphincter electromyogram (EUSE) activity, evoked by a single or by repetitive pelvic nerve stimulation, in 30 anesthetized rats was recorded with/without bladder saline distension. Without saline distension (0 cmH2O), a single pulse nerve stimulation evoked a single action potential in the reflex activity, whereas repetitive pelvic stimulation and saline distension (6?20 cmH2O) both elicited a long-lasting reflex potentiation (20.05 ± 3.21 and 75.01 ± 9.87 spikes/stimulation, respectively). The saline distension-induced pelvic nerve-to-EUS reflex potentiation was abolished by D-2-amino-5-phosphonovalerate [APV; a glutamatergic N -methyl-D-aspartic acid (NMDA) receptor antagonist; 100 ?M, 10 ?l, 1.72 ± 0.31 spikes/stimulation] and attenuated by 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo (F) quinoxaline [NBQX; a glutamatergic α-amino-3-hydroxy-5-methyl-4-isoxazoleproprionate (AMPA) receptor antagonist; 100 ?M, 10 ?l, 26.16 ± 7.27 spikes/stimulation], but was not affected by bicuculline (a GABAergic antagonist; 100 ?M, 10 ?l, 53.62 ± 15.54 spikes/stimulation). Intrathecal administration of glutamate (31.12 ± 8.25 spikes/stimulation, 100 ?M, 10 ?l) and NMDA (26.25 ± 4.12 spikes/stimulation, 100 ?M, 10 ?l) both induced a long-lasting pelvic nerve-to-EUS reflex potentiation without saline distension, which was similar to the findings observed from saline distension only. The duration of the contraction wave of the urethra was elongated by the saline distension-induced pelvic nerve-to-EUS reflex potentiation, whereas the peak pressure of the contraction wave was not affected. Our findings suggest that saline distension in the bladder elicits a pelvic nerve-to-EUS reflex potentiation and the glutamatergic mechanism contributes to the presence of such a reflex potentiation.
URI:	https://ir.csmu.edu.tw:8080/handle/310902500/3613 http://dx.doi.org/10.1152/ajprenal.00443.2006
關聯:	Am J Physiol Renal Physiol. 292, 1791-1801.
显示于类别:	[醫學系] 期刊論文

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