氫氧磷灰石（Hydroxyapatite，簡稱HA）具有生物相容性且能和齒槽骨產生化學性結合，並且能被吸收轉變成新生骨質，常被使用在口腔外科與牙周病科做為骨質缺損處之填充物。本研究就中山醫學院牙醫學系自行合成之HA，利用組織培養方法，探討其細胞毒性作用，評估臨床使用之可行性。本實驗所採用之細胞株為中國倉鼠肺纖維母細胞（V79 cells）， 在60 mm培養皿中植入1 X 10穩茞茩M培養24小時後，加入HA 20顆共同培養四天，並以不加HA者為對照組，分別計算細胞生長數目，以trypan blue染料染色法計算細胞存活率，另在位相差顯微鏡及掃描式電子顯微鏡下觀察細胞形態、生長情形。結果顯示， 實驗組與對照組在細胞生長數目、存活率上並無顯著差異（p > 0.05），且在位相差顯微鏡下可觀察到V79細胞型在HA顆粒底部附著， 再以電子顯微鏡進一步觀察證實V79細胞確可附著在HA上。綜合本實驗結果，本系合成之HA對V79細胞並不具有細胞毒性。
Hydroxyapatite (HA) is biologically compatible and has been used in the treatment of periodontal osseous defects and in ridge augmentation. The purpose of this study was to investigate the cytotoxic effect of synthetic HA synthetic by School of Dentistry, Chung Shan Medical & Dental College. The cytoxicity was tested using a tissue culture method with a cell line derived from chinese hamster lung fibroblasts (V79 cells). About 1 x 10 V79 cells were plated on each 60 mm petri dish. After 24 hours, 20 pieces of HA were added to the petri dishes and co-cultrued for a further 4 days. the cytotoxicity of HA was analyzed using the cell growth curve, cell viability, phase constrast microscopy (PCM) and scanning electron microscopy (SEM). The results showed that there was no difference in the viability or cell growth curve untreated V79 cells and V79 cells co-cultured with HA (p < 0.05). PCM revealed that V79 cells could attach to the bottom of HA. Further observation with SEM showed that V79 cells really attached to the surface of HA. In conclusion, synthetic HA is biocompatible with V79 cells.
