近年來，各項生化分析工具的微小化， 使得分析物消耗量及分析時間大幅減少，已成 為一股新的研究趨勢 。其中毛細管電泳技術 相較於傳統平板膠電泳而言，具有分析迅速、 樣品需要量、分析效率高及可自動化之優勢， 而結合微小化技術之毛細電泳晶片，更是樣品 分析上的一大利器。在本研究中，採用 PE/PET 為電泳晶片基材，採用切割的方式製作出微管 道，再配合高溫護貝技術，進行晶片接合。換 句話說，本研究將原來三度空間製程，簡化成 兩度空間製程，在割製原型完全不需要考慮到 管道深度的問題，而有簡化製程、大幅縮短晶 片製作時間、以及降低製作成本之優點。其 次，針對塑膠薄型電泳晶片做性質上的探討， 最 後 根 據 塑 膠 薄 型 電 泳 晶 片 易 於 裁 剪 的 特 性，製作一體成型的電灑法塑膠噴頭，而衍生 出塑膠微電灑質譜晶片。總結本實驗之製程， 皆以簡單、迅速為取向；期待能夠以更短的時 間、更少的成本來大量化的製作出高效率之生 醫分析工具。
Over the last years, the miniaturization of biochemical analytical tools has become an expanding field. Indeed, such devices allow decreasing both the consumption of analytes and the duration of analyses. Comparing to traditional slab gel electrophoresis, capillary electrophoretic methods show an advantage of fast analysis, low sample consumption, high analyzing efficiency, and automation. Recently the developments of chip-based separation devices are increased speed and reliability at reduced sample consumption and cost, particularly microchip electrophoresis. In this study, a polymeric thin-film microchip for electrophoresis and mass spectrometry was developed. The micro channel is fabricated by micro blade method in PE/PET films, and then sealed by lamination. Because the fabrication without considering the depth of micro channel, it reduces manufacture process from the 3-dimention to 2-dimention in CE chip fabrication. By this way, we take advantage of simply fabrication process and less fabricating time and cost. The characterization of the polymeric thin-film electrophoresis microchip was tested and nanoESI chip was developed according to easy cutting, and sealing of the thin film. The merits and limitations of those approaches are discussed. To summarize, depending on simplification and rapidity; we looking forward to fabricate high efficiency analytical tools with less time and cost. Therefore, there is also potential for development of disposable diagnostic systems for biomedical applications.
