建立保健食品在減緩糖尿病誘發血管病變功能之評估方法

中山醫學大學機構典藏 CSMUIR > 醫學院 > 生化微生物免疫研究所 > 研究計劃 > Item 310902500/3110

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/3110

Title:	建立保健食品在減緩糖尿病誘發血管病變功能之評估方法
Authors:	王朝鐘;黃建寧;何協勳;陳香梅;杜昌哲 Wang, Chau-Jong;Huang, Chien-Ning;Hsieh-Hsun Ho;Chen, Hsiang-Mei;Tu, Chang-Che
Contributors:	中山醫學大學生物化學研究所
Keywords:	糖尿病;血管病變;平滑肌細胞
Date:	2008
Issue Date:	2010-12-07T09:24:11Z (UTC)
Abstract:	現今全球普遍面臨糖尿病盛行率不斷增加，死亡率提高，血糖控制不佳，合併多重的併發症，這些問題在開發中國家尤其嚴重，而台灣恰好是其中一員。健康食品開發勢趨成熟，市面上所流通之保健食品亦不計其數。然而，獲得衛生署所認可之健康食品卻僅有四十多種，可見以現有之健康食品功能性規範，並不足以滿足日趨增加的健康食品市場。而在現今糖尿病患者日益增加的情況下卻沒有針對已患有糖尿病之國人，減緩相關併發症之功能進行規範之訂定。因此本方法之建立，將有助於糖尿病患者減緩血管病變之作用。在本實驗中先以高糖處理血管平滑肌細胞 (A7r5) 並觀察細胞增生、位移及受損的情形。結果顯示高糖能引起血管平滑肌細胞增生及位移。接著我們使用Wistar大白鼠，並以糖尿病誘導物STZ (streptozotocin) 65mg/kg 進行腹腔注射，確認血糖濃度增加後以purina動物飼料餵食24週後，進行血液分析、生化分析、H&E染色及組織免疫染色等實驗方法，結果發現血液中糖化血色素 (HbA1c) 、血脂肪 (LDL) 、TG及 cholesterol 均明顯增加，肝功能指標 (AST、ALT) 也異常升高，血管內皮細胞明顯受到傷害，平滑肌細胞亦發生增生及位移，顯示在此模式下可誘導早期之atherogenesis，進一步以TZD、Zocor及保健食品洛神花水萃取物處理，均顯著的抑制早期之atherogenesis。另以一種小鼠C57BL/6模式，餵食diabetogenic diet (Dd) 14週後，其血清生化值 (HbA1c、TG及cholesterol)增加較不明顯，以及血管atherogenesis之早期現象，亦較不顯著增加，因此本研究提出評估延緩糖尿病致早期血管病變之方法為 (1) 25 mM glucose 致血管平滑肌細胞增生及位移; (2)以STZ 65mg/kg 致Wistar大白鼠血糖增加後餵食purina動物飼料24週，合併以上兩種模式可評估保健食品之延緩作用。 Today, the population of diabetes is increase and the mortality rate is also enhancing in the whole world. Type II diabetes patient is getting young, the blood sugar control is not good, multiple complication resulting in the family, social acute question and the cost huge resources. Many countries exists these serious problems, including Taiwan. The development of healthy food is mature gradually and commercial health foods are more and more. However, health food has the approval of DOH only about 40 items. So far, absence of the functional standard is insufficient for the healthy food marketplace needs. Hence, we establish the standard protocol of diabetes complication in retarding is necessary. This method will be helpful to confirm that the diabetes retard on blood vessel pathological change. In this study, we explored the differentiation and migration of smooth muscle cells (A7r5) that treated high glucose (25 mM). We found that high glucose markedly induced A7r5 cells differentiation and migration. Next, we further investigated this effect and used animal model of STZ (streptozotocin)-induced diabetes in Wistar rats by intraperitoneal inject (IP) method. After 24 weeks, we showed that data of HbA1c (glycated hemoglobin), LDL (low-density lipoprotein), TG (triglyceride) and cholesterol have notable induction by blood analysis, biochemical analysis, H&E stain, IHC analysis etc.. Furthermore, liver functional marker (AST and ALT) were also increase. In addition to, endothelial cell of rats blood vessel were damaged and smooth muscle cell were differentiated and migrated. The data indicated that the model could induce atherogenesis in early stage. When TZD, Zocor and HSE were administered for rats, all have obvious inhibition of atherogenesis in early stage. We used another animal model; C57BL/6 mice fed diabetogenic diet during 14 weeks. Neither the data of HbA1c, TG and cholesterol nor blood vessel pathology were any effect. Based on the research, we were provided with two methods to evaluate delay atherogenesis of diabetes in early stage: (1) 25 mM glucose-induced differentiation and migration of smooth muscle cell; (2) STZ (streptozotocin)-induced diabetes in Wistar rats. We suggest that the role of healthy food in retarding needs to be assessed according to combine above two methods.
URI:	https://ir.csmu.edu.tw:8080/handle/310902500/3110
Appears in Collections:	[生化微生物免疫研究所] 研究計劃

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