| Abstract: | 為了探討電針刺激的神經機制,設計下列兩組實驗分別觀察電針刺激對心血管系統以及泌尿系統的影響。 前半部電針刺激影響循環系統的實驗以各種不同強度及頻率的電針刺激合谷(Li-4)及足三里(St-36),記錄其對血壓及腎上腺交感神經的影響。其結果分述如下:(1).電針刺激足三里對血壓及腎上腺神經活性均不產生任何影響。但是,強度為 5xT 或更高的電針刺激合谷,在刺激期間能顯著地升高血壓及腎上腺神經活性。(2).低頻(3 Hz)和高頻(30 Hz)的電針刺激對於血壓及腎上腺神經活性的影響型明顯不同:低頻電針刺激引起一張力性(Tonic)變化,而高頻電針刺激則為相位性(phasic)變化。(3).同時以相同頻率的電針刺激兩邊合谷,血壓及腎上腺神經活性變化的型式和強度,與單邊刺激似無不同,但其到達最大效用的時間則較為縮短。(4).同時以低頻和高頻電針刺激兩邊合谷,此種合併式的電針刺激所引發的反應,則兼具有高頻及低頻刺激的特性。(5).電針刺激對血壓及腎上腺神經活性的影響不會受腎上腺切除影響,但卻會被靜脈注射Regitine,一種腎上腺阻斷劑所減弱或阻斷。
The following work was designed to investigate the neural mechanism of electroacupuncture (EA). Two sets of experiments were carried out to determine the effect of EA on circulatory, urinary and the nervous systems. Response of arterial blood pressure (BP) and adrenal nerve activity (ANA) to EA were studied in anesthetized rats and the results are summarized as following: 1. At Tsusanli, EA was of no effect. However, at Hoku, an elevation of BP and ANA was elicited during EA when the intensity was 5xT or higher. 2. The pattern of pressor effects caused by EAs with frequency of 3 and 30 Hz were different, i.e., a tonic effect was elicited with frequency of 3 Hz, while a phasic one was induced with 30 Hz. 3. The pressor effects elicited by bilateral EAs were similar to that of unilateral ones, except the latency to reach the maximal effect was shortened. 4. A combination of both phasic and tonic effects on BP and ANA was obtained when EA at Hoku on both sides simultaneously with different frequencies (3 and 30 Hz) on each side. 5. The pressor effects elicited by EA was not affected by adrenalectomy, but abolished by regitine, an alpha adrenergic blocker. To address the effect of EA on bladder activity, responses of rhythmic micturition contraction (RMC), urine excretion (UE), BP, sympathetic renal nerve activity (RNA) and pelvic parasympathetic nerve activity (PNA) elicited by EA were investigated in urethane-anesthetized rats. The results are summarized as following: 1. Elongation of RMC cycle and increase in UE associated with the elevation of BP and RNA were elicited during EA at Hoku. 2. The pressor response induced by low frequency EA (LFEA) was different from that by high frequency EA (HFEA), i.e., a tonic effect was elicited by LFEA, while a phasic one was induced by HFEA. These results implicate that (1) EA at Hoku with different stimulation frequency may excite a distinct mechanism to activate the sympathetic nervous system, (2) EA with stimulation intensity of 5xT or higher can increase and maintain BP, ANA and RNA as well as elongate RMC. (4) EA at Hoku may elevate BP and ameliorate the hyperactive bladder in clinical therapy. |
