本研究探討不同表面粗糙度的Ti及Ti合金對類造骨細胞（U-2 OS）貼附及增殖的影響。利用表面粗度儀測量表面粗糙度，利用原子力顯微鏡觀察材料表面立體形態。細胞成長方面，利用MTT分析法評估細胞貼附（二小時）數量，利用台盤藍排除法計算細胞增殖（三天）數目，利用結晶紫染色技術及掃瞄式電子顯微鏡觀察細胞的形態。本研究結果顯示，表面粗糙度會影響U-2 OS細胞在Ti及Ti合金表面初期的貼附數目及形態，材料種類會影響U-2 OS細胞在材料表面的增殖行為。 The effect of surface roughness of Ti and Ti6Al4V alloy on the adhesion and proliferation behavior of osteoblast-like cell (U-2 OS) was investigated. The surface roughness (Ra), measured by profilometry. 3-D surface topography was measured using atomic force microscope, respectively. MTT assay and trypan blue exclusion method were used to calculate the number of cells attached after 2 h and 3 d incubation, respectively. Crystal violet stain technique and scanning electron microscope were used to characterize the cell morphology after 2 h and 3 d incubation. The results of this study showed that surface roughness had a significant effect on the cell morphology and cell adhesion number after 2 h incubation, while the material had a significant influence on the cell proliferation number after 3 d incubation
