| Abstract: | 臨床上有不同之材料應用於牙髓斷髓術之治療。本研究之目的乃比較不同之配方下，這些材料對於L929 細胞株之毒性反應。研究材料乃採用下面幾組配方：1.Zinc oxide powder, eugenol 和formocresol (FC). 2. Zinc oxide powder,eugenol 和glutaraldehyde(Glu). 3. Zinc oxide powder, eugenol 和ferricsulfate (FeS). 4. Calcium hydroxide, distilled water 和formocresol. 5.Calcium hydroxide (Ca(OH)2), distilled water 和glutaraldehyde. 6. Calciumhydroxide, distilled water 和ferric sulfate.。所有材料調製完成後浸泡於細胞培養液中，並將之置備濃度為10, 20, 40 和80 μl/ml 之溶液，細胞粒線體呈色反應(MTT assay)用於偵測材料對於L929 細胞株之存活率。結果以one way ANOVA統計分析比較其存活率，事後分析以Student-Newman-Keul's test 比較。結果顯示六種配方之存活率因所配置之濃度高低有不同之大小，濃度高時細胞之存貨率下降(p<0.05)。較高之細胞存活率出現於第3,5,和6 組。結語：臨床上使用此材料十可以考慮使用下面之組合Zinc oxide powder, eugenol 和ferricsulfate; Calcium hydroxide, distilled water 和glutaraldehyde; 或Calciumhydroxide, distilled water 和ferric sulfate。近一步研究則可以於日後進行臨床之研究比較。
A number of pulpotomy dressing materials have been applied clinically with various rates of success. The purpose of the present study was to evaluate the toxicity of different medicaments on treated L929 cells. The puplotomy preparations were grouped as follows: 1. Zinc oxide powder, eugenol and formocresol (FC). 2. Zinc oxide powder, eugenol and glutaraldehyde(Glu). 3. Zinc oxide powder, eugenol and ferric sulfate (FeS). 4. Calcium hydroxide, distilled water and formocresol. 5. Calcium hydroxide (Ca(OH)2), distilled water and glutaraldehyde. 6. Calcium hydroxide, distilled water and ferric sulfate. All mixed materials were dissolved in medium and diluted to 10, 20, 40 and 80 .mu.l/mlconcentrations. A cell colorimetric assay (MTT) was used to detect the viability of L929 cells. Results were compared using one way analysis of variance (ANOVA). Differences in treatment means were analyzed using Student-Newman-Keul's test and were considered significant at p <0.05. The survival rate of treated L929 cell showed statistical differences as the concentrations of the pulpotomy materials increased (p<0.05). The highest survival rates were found in groups 3, 5 and 6. Conclusions: It is recommended that low toxicity formulas such as Zinc oxide powder, eugenol and ferric sulfate; Calcium hydroxide, distilled water and glutaraldehyde; or Calcium hydroxide, distilled water and ferric sulfate be used clinically as pulpotomy dressing materials. Further research with randomized clinical trials is needed to verify these clinical success rates. |
