三氧礦聚合物(簡稱MTA, Mineral Trioxide Aggregate)常被用於作根尖充填之材
料，臨床上由於硬化時間過長與成本上略高的問題，常為使用者抱怨。本研究室
過去將此材料做過一探討，並已有能力自行合成，因此本年度之研究主要目的為
將先前計劃已配製出之材料進一步作生物學上之相容性測試，以了解本材料之生
物相容性。本研究之實驗方法與分析包括以MTT分析細胞之毒性，以DNA 裂解
觀察材料對於細胞之基因毒性，以RTPCR測試發炎蛋白COX-2是否出現，以西
方墨點分析細胞內訊息蛋白之反應。結果顯示本材料對於細胞之生長沒有影響，
且於西方墨點反應中出現ERK kinase蛋白，證據顯示不具有細胞毒性；於DNA
裂解反應中伊發現沒有DNA破裂出現，顯示未具有基因毒性；而細胞之發炎反
應中，雖有見到COX-2 之出現，但我們認為此為正常之細胞反應。結論：本研
究室所配製之MTA具有細胞相容性，日後可再進行活體內之試驗和臨床研究。
MTA( mineral trioxide aggregate) is a root end filling material. Clinically it existed
long setting time and expansive. Our laboratory has developed a novel MTA last year,
and its physical and chemical properties has discussed. The purpose of the present
study was to evaluate this novel material its biocompatibility in vitro. Material and
method: In present study, the MTT assay was to evaluate the L929 and U2OS cell
viability. The DNA fragmentation assay was to evaluate the genotoxicity of material
contact with cells. The RT-PCR assay was to detect the COX-2 expression and other
interleukins. The western blot assay was to detect the MAPK kinase expression.
Results: the present result showed that this novel material existed cellular
biocompatibility accomplained with the ERK kinase expression. The DNA breakage
of treated cells did not exist. The COX-2 expression were shown in all groups.
Conclusion : This novel material is biocompatibility with cells. The further study
should be focus on the in vivo test and clinical test.
