中山醫學大學機構典藏 CSMUIR:Item 310902500/2852
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    請使用永久網址來引用或連結此文件: https://ir.csmu.edu.tw:8080/ir/handle/310902500/2852


    題名: 鼷鼠感染廣東住血線蟲造成經脫髓鞘之致病機轉
    Pathogenesis of Demyelination in Mice Caused by Angiostrongylus Cantonensis
    作者: 賴世展
    Lai, Shih-Chan
    貢獻者: 中山醫學院醫學系
    關鍵詞: 廣東住血線蟲;腦膜腦炎;腦膜炎;脫髓鞘
    Angiostrongylus cantonensis;Meningoencephalitis;Meningitis;Demyelination;CNP
    日期: 2008
    上傳時間: 2010-11-25T03:11:20Z (UTC)
    摘要: 廣東住血線蟲(Angiostrongylus cantonensis)
    引起中樞神經系統嚴重感染，包括慢性脫
    髓鞘(demyelination)疾病。關於髓鞘疾病指
    標性酵素2', 3'-cyclic nucleotide
    3'-phosphodiesterase ( CNP )在生物學所扮
    演的角色需要被研究。本研究以BALB/c
    鼷鼠感染廣東住血線蟲誘發脫髓鞘的腦組
    織和腦脊髓液中CNP 的表現量，闡明廣東
    住血線蟲誘發脫髓鞘在6 個不同的時間點
    CNP 的變化，同時以定量反轉錄酶聚合酶
    鏈反應( reverse transcription-quantitative
    competitive polymerase chain reaction ，
    RT-qPCR ) 和利用西方轉漬法(Western
    blotting analysis)測定CNP 的含量。結果顯
    示在嚴重階段的腦膜腦炎或腦膜炎中
    CNP 在鼷鼠腦脊髓液中與控制組相比較下有顯著向上調節趨勢。CNP 特殊的標號
    定位到神經膠質細胞的細胞質，這些結果
    顯示中樞神經系統感染廣東住血線蟲誘發
    脫髓鞘與CNP 相關聯。因此推斷從鼷鼠感
    染廣東住血線蟲誘發脫髓鞘產生CNP 在
    腦脊髓液中的量顯示中樞神經髓鞘被破
    壞，因此在腦脊髓液的嗜伊紅性腦膜炎可
    以測定CNP 而應用在臨床上的管理。
    Angiostrongylus cantonensis causes severe
    central nervous system (CNS) infection,
    including a chronic demyelinating disease.
    The biological role of 2’, 3’-cyclicnucleotide 3’-phosphohydrolase (CNP) in
    myelin-related disease requires investigation.
    We investigated the level of CNP in the
    brain tissues and cerebrospinal fluid (CSF)
    of mice with demyelination caused by A.
    cantonensis. To elucidate the changes of
    CNP on demyelination caused by A.
    cantonensis were taken at six different time
    points, at 5–25 days post-inoculation. CNP
    was investigated by reverse
    transcription-quantitative polymerase chain
    reaction and Western blotting. CNP in
    mouse CSF was significantly upregulated in
    the severe phase of meningoencephalitis or
    meningitis as compared with controls.
    Specific labeling was localized to the
    cytoplasmic membrane of glial cells. These
    results showed that CNP was associated
    with A. cantonensis-induced demyelination
    of CNS. We conclude that CNP in the CSF
    from mice with angiostrongyliasis
    demyelination is an indicator of destruction
    of myelin in the CNS, and the measurement
    of CNP in the CSF of eosinophilic
    meningitis may be useful in clinical management.
    URI: https://ir.csmu.edu.tw:8080/handle/310902500/2852
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