廣東住血線蟲(Angiostrongylus cantonensis)
引起中樞神經系統嚴重感染，包括慢性脫
髓鞘(demyelination)疾病。關於髓鞘疾病指
標性酵素2', 3'-cyclic nucleotide
3'-phosphodiesterase ( CNP )在生物學所扮
演的角色需要被研究。本研究以BALB/c
鼷鼠感染廣東住血線蟲誘發脫髓鞘的腦組
織和腦脊髓液中CNP 的表現量，闡明廣東
住血線蟲誘發脫髓鞘在6 個不同的時間點
CNP 的變化，同時以定量反轉錄酶聚合酶
鏈反應( reverse transcription-quantitative
competitive polymerase chain reaction ，
RT-qPCR ) 和利用西方轉漬法(Western
blotting analysis)測定CNP 的含量。結果顯
示在嚴重階段的腦膜腦炎或腦膜炎中
CNP 在鼷鼠腦脊髓液中與控制組相比較下有顯著向上調節趨勢。CNP 特殊的標號
定位到神經膠質細胞的細胞質，這些結果
顯示中樞神經系統感染廣東住血線蟲誘發
脫髓鞘與CNP 相關聯。因此推斷從鼷鼠感
染廣東住血線蟲誘發脫髓鞘產生CNP 在
腦脊髓液中的量顯示中樞神經髓鞘被破
壞，因此在腦脊髓液的嗜伊紅性腦膜炎可
以測定CNP 而應用在臨床上的管理。
Angiostrongylus cantonensis causes severe
central nervous system (CNS) infection,
including a chronic demyelinating disease.
The biological role of 2’, 3’-cyclicnucleotide 3’-phosphohydrolase (CNP) in
myelin-related disease requires investigation.
We investigated the level of CNP in the
brain tissues and cerebrospinal fluid (CSF)
of mice with demyelination caused by A.
cantonensis. To elucidate the changes of
CNP on demyelination caused by A.
cantonensis were taken at six different time
points, at 5–25 days post-inoculation. CNP
was investigated by reverse
transcription-quantitative polymerase chain
reaction and Western blotting. CNP in
mouse CSF was significantly upregulated in
the severe phase of meningoencephalitis or
meningitis as compared with controls.
Specific labeling was localized to the
cytoplasmic membrane of glial cells. These
results showed that CNP was associated
with A. cantonensis-induced demyelination
of CNS. We conclude that CNP in the CSF
from mice with angiostrongyliasis
demyelination is an indicator of destruction
of myelin in the CNS, and the measurement
of CNP in the CSF of eosinophilic
meningitis may be useful in clinical management.
