在骨性關節炎中分析基質金屬蛋白水解脢-酵素活性變化以探討關節鏡手術的治療效果機轉

中山醫學大學機構典藏 CSMUIR > 醫學院 > 醫學系 > 研究計劃 > Item 310902500/2830

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题名:	在骨性關節炎中分析基質金屬蛋白水解脢-酵素活性變化以探討關節鏡手術的治療效果機轉 Expression Changes of Gelatinases in Human Osteoarthritic Knee and the Arthroscopic Debridement
作者:	呂克修 Lu, Ko-Hsiu
贡献者:	中山醫學院醫學系
关键词:	骨性關節炎;基質金屬蛋白酵素;酵素活性;關節鏡 Osteoarthritis;Matrix metalloproteinase;Enzyme activity;Arthroscopy
日期:	2003
上传时间:	2010-11-25T03:10:55Z (UTC)
摘要:	Purpose: To quantify the expression changes of gelatinase-A & B (matrix metalloproteinase-2 & 9, MMP-2 & 9) in a series of chondral, meniscal, and synovial cultures of knee osteoarthritis (OA) for investigation of the possible roles of the cartilage, menisci and synovia and the efficacy of arthroscopic dbridement. Type of Study: Case series. Methods: In consecutive 43 patients with knee OA to receive arthroscopic dbridement, we examined the amount of MMP-2 & 9 in a series of chondral, meniscal and synovial cultures. We also compared the gene expressions of MMP-2 & 9 and membrane-type 1 MMP (MT1-MMP) in the chondral, meniscal, and synovial cultures using reverse transcription polymerase chain reaction (RT-PCR). Results: Latent & activated forms of MMP-2 were produced in all series of chondral, meniscal, and synovial cultures and their activities in lesional cultures were significantly higher than that in paralesional ones (P < .001). Moreover, the latent form of MMP-9 (proMMP-9) appeared in 29 of 37 series of synovial cultures and in 13 of 40 series of meniscal cultures. In meniscal cultures after 24 hours incubation and synovial cultures after 3 and 24 hours incubation, the activity of proMMP-9 in lesional cultures was significantly higher than that in paralesional ones (P < .001). The activated form of MMP-9 appeared in 10 of 37 series of synovial cultures and its activity in lesional cultures was significantly higher than that in paralesional ones (P < .05). Furthermore, MMP-2, -9 and MT1-MMP mRNA levels of lesional areas also showed the increased expression in RT-PCR. Conclusions: Our data confirm that tissue repair of OA is ascribable to enzymic digestion of the ECM ex vivo. When technically appropriate, arthroscopic debridement for the pathologic lesions of OA, such as meniscal tears, chondral lesions, and hypertrophic villi, may be beneficial to the process of early cases. Still, it should be carefully studied for its overall effect and mechanism in vivo.
URI:	https://ir.csmu.edu.tw:8080/handle/310902500/2830
显示于类别:	[醫學系] 研究計劃

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呂克修.pdf	國科會計劃報告書	239Kb	Adobe PDF	645	检视/开启

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