為了瞭解在台灣地區各年齡層的人受人類多瘤性病毒感染情形,我們收集869件尿液進行分析。我們利用PCR、Southern blot及DNA sequencing等方法測試尿液檢體中之人類多瘤性病毒之DNA並鑑定其基因型。我們的結果顯示幼稚園兒童之尿液檢體只有1%(1/104)有JC病毒存在。此外,國小學童(3-7歲)為3.24%(7/216)、國中及高中學生(8-13歲)為6.51%(17/261)、大學生(20-30歲)為13.3%(10/75)、成人(31-50歲)為33.3%(5/15)、中年人為41.7%(5/12)及老年人(61-70歲以上)為53%(98/186)JC病毒陽性。JC病毒基因型之總比例分別為CY 44.8%(64/14)及台灣一號55.2%(79/143)。此結果顯示年齡愈大者排放人類多瘤性病毒之比例愈高,此現象可能與人類免疫有關。此外,台灣一號及CY之JC病毒為台灣盛行之基因型。這兩種基因型之生理特性及致病機轉有待更進一步研究。
To investigate the prevalence of human polyomavirus in normal population with various ages in Taiwan, 869 urine sample were collected for analysis. Polymerase chain reaction (PCR), Southern blot and DNA sequencing were performed to detect the viral DNA and genotype identification. The results show that 1% (1/104) of age range 3-7, 3.24% (7/216) of age range 8-13, 6.51% (17/261), 13.3% of age range 20-30, 33.3% (5/15) of age range 31-50, 41.7% (5/12) of age range 51-60 and 53% (98/186) of age greater than 61 were JCV positive. The results indicate that the incidence of JC viruria is increased with age, which may be correlated with immunity. In addition, CY (44.8%) and TW-1 (55.2%) are the predominant genotypes of JCV prevalent in Taiwan. The relationship between viral genotype and pathogenesis needs to be further investigated.
