各種蜂膠萃取物生物活性與口腔癌化學預防特性之研究

中山醫學大學機構典藏 CSMUIR > 健康管理學院 > 營養學系暨碩士班 > 研究計劃 > Item 310902500/2753

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/2753

Title:	各種蜂膠萃取物生物活性與口腔癌化學預防特性之研究 Study on the Biological Activities and Chemoprotective Properties on Oral Cancer of Various Extracts of Propolis
Authors:	王進崑 Wang, Chin-Kun
Contributors:	中山醫學院營養系
Keywords:	蜂膠;Cox-2抑制劑;前列腺素;消化;抗氧化性;酚類化合物 Propolis;Cox-2 inhibitor;Prostagladin;Digestion;Antioxidation;Phenolics
Date:	2003
Issue Date:	2010-11-24T07:48:51Z (UTC)
Abstract:	蜂膠為具保健功能之天然物，但對於各種不同蜂膠萃取物其消化前後之組成與活性則缺乏相關之研究，本研究針對不同蜂膠萃取物((甘油、水以及30％、60％、95％的酒精水溶液等蜂膠萃取物)消化前後組成及生物活性進行探討。首先瞭解不同萃取液之組成與生物活性之差異性，再探討經過人工胃液以及先經人工胃液再經人工腸液的消化後其組成和生物活性的變化。由於酚類化合物為蜂膠之主要活性貢獻物質，因此以酚類化合物之組成變化來比較蜂膠消化前後的差異，而有關生物活性的評估則包含了總抗氧化能力、還原力、自由基的清除以及對COX-2表現與PGE2之生成的抑制情形。本研究之結果得知，95％與60％的蜂膠乙醇水溶液萃取物的組成和生物活性相近，而水和30％蜂膠乙醇水溶液萃取者則相類似，但其生物活性較95％與60％乙醇兩者水溶液萃取者差，而甘油萃取者則介於兩大組之間。經HPLC分析酚類化合物得知，蜂膠萃取物經消化處理後，酚類化合物之含量隨著萃取溶劑中乙醇含量比例降低而顯著減少。而活性表現方面(包括總抗氧化能力、清除過氧化氫、DPPH、還原能力以及抑制COX-2的表現與 PGE2的生成)，經消化處理後各種翠取物之活性表現不若原始萃取物；而消化處理前後，皆以95％與60％蜂膠乙醇水溶液萃取者之功效較佳；在螯和亞鐵離子和清除超氧陰離子方面，在未經消化處理時，以水萃取者之效果較佳，但經消化處理後，水萃取者效果反而有最明顯的降低，比較HPLC之分析結果可知，滯留於0-50分鐘具有較高極性之單寧物質可能為其主要貢獻物質。 Propolis is a very popular nature health food currently. However, the composition and biological actives of various propolis extracts and their digested products are still deficient. Therefore, the composition and biological actives of propolis extracts and their digested products will be discussed in this study. 95%EtOH, 60% EtOH, 30% EtOH, aqueous and glycerol extracts of propolis were used in this study. Phenolics are found to be the major available physiological contributor of propolis, the phenolic composition and biological actives in various propolis extracts and their digested products will be determined. In addition, the change of phenolic composition and biological actives of the extracts after gastric digestion or gastrointestinal digestion were also determined. Biological evaluations include total antioxidant capacity, reducing power, free redical scavenging efficiency, suppression efficiency for COX-2 expression and PGE-2 formation. Result revealed that 95% HEtOH and 60% HEtOH extracts of propolis showed similar phenolic components and biological actives. Both 30% HEtOH and aqueous extract of propolis showed less levels of phenolics and lower biological actives than that of 95% HEtOH and 60% HEtOH extracts. The phenolic contents and biological effects of glycerol extract are between the above two groups. By the analysis of HPLC, phenolics were greatly decreased and changed, especially in 30% HEtOH and aqueous extracts. The digestion treatment greatly decreased the biological activities of various propolis extracts. Both 95% HEtOH and 60% HEtOH extracts showed better biological effects (total antioxidant capacity,H2O2 scavenging, reducing power, DPPH, free redical scavenging and suppression efficiency for COX-2 expression and PGE2 formation) than the other extracts before and after digestion. The effects on chelating ferrous ion and superoxide anion scavenging were only shown by the original aqueous extract. However, digestion of aqueous extract greatly reduced both effects. By the results of HPLC on phenolics compounds, the tannin-like materials with high polarity in the range of 0-50 minutes were probably the main contributor for cheating ferrous ions and scavenging superoxide anion.
URI:	https://ir.csmu.edu.tw:8080/handle/310902500/2753
Appears in Collections:	[營養學系暨碩士班] 研究計劃

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