子計畫一---自體免疫疾病中蛋白質精胺酸甲基化之蛋白質體研究(I)

中山醫學大學機構典藏 CSMUIR > 醫學科技學院 > 生物醫學科學學系暨碩士班 > 研究計劃 > Item 310902500/2685

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题名:	子計畫一---自體免疫疾病中蛋白質精胺酸甲基化之蛋白質體研究(I) Proteomic Analyses of Methylarginine Modification in Autoimmune Diseases (I)
作者:	李娟 Li, Chuan
贡献者:	中山醫學大學生物醫學科學學系
日期:	2005
上传时间:	2010-11-05T10:48:32Z (UTC)
摘要:	在此研究計劃中，我們想要探討蛋白質胺酸甲基化（分為對稱型或對稱型甲基胺酸）和自體免疫疾病的關係。許多蛋白質包含甲基胺酸，如：fibrillarin、部分的hnRNP蛋白、myelin basic protein以及SmD1、D3蛋白，而這些蛋白也已被發現是許多自體免疫疾病的自體免疫抗原。在這個計劃中，我們首先從紅斑性瘡病人中取得anti-Sm和anti-RNP自體免疫抗體血清及獲取其他的免疫抗體血清，尋找抗血清中是否有區分蛋白質胺酸甲基化之抗體，再用蛋白質體學方法找出有甲基化自體免疫抗原。我們在一位病人血清作西方點墨法時，發現低甲基化之細胞萃取蛋白和正常甲基化組比較，有調訊號減低情況。我們將相關蛋白點挖出，以蛋白脢水解後作質譜分析，有二蛋白質含有典型胺酸甲基化蛋白質中出現之 RGG序，是否此自體免疫抗體所辨的自體免疫抗原被辨認的位置與胺酸甲基化有關正在研究中。 In this project we are going to find out the relationship between protein methylation, mostly protein N-arginine methylation to form symmetric or asymmetric NG, NG –dimethylarginines (sDMA or aDMA), and autoimmune disease. Many methylarginine containing proteins such as fibrillarin, several hnRNP, myelin basic protein and SmD1 and D3 are known to be autoantigens of different autoimmune diseases. Sm protein D1 and D3 were reported to contain symmetric di-methylarginines and a few different anti-Sm autoantisera recognized only the sDMA peptide of SmD1 and D3 but not unmethylated or asymmetric dimethylarginine peptides. We thus examined if the anti-Sm sera from local SLE patients also preferentially recognize the methyl-modified Sm D proteins and if there are other proteins that can be differentially recognized by the anti-Sm sera due to their methylation states. We treated HeLa cells withadenosine dialdehyde (AdOx), an inhibitor of protein methylation. Anti-Sm autosera from three different SLE patients were used in western blot analyses of AdOx-treated (proteins presumably at hypomethylation state) and untreated (proteins at normal methylation states) HeLa cell extracts. Reduced ignalsbetween molecular mass of 18 to 21 kDa and about 31 kDa were consistently detected from cell extracts treated with AdOx compared to the ones without AdOx treatment by one-dimensional SDS-PAGE analyses. However, there were no significant differences between the signals orresponding to SmD1 in samples of different methylation status. By two-dimensional electrophoresis, the differentially detected signals were further pinpointed and putative spots were picked by comparing the western signals with the protein sypro ruby stain, and digested by trypsin. The peptide fragments were analyzed by MS and MS/MS analyses.
URI:	https://ir.csmu.edu.tw:8080/handle/310902500/2685
显示于类别:	[生物醫學科學學系暨碩士班] 研究計劃

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