環境危險因子、代謝基因之多型性與子宮頸前癌和子宮頸癌之相關性探討

中山醫學大學機構典藏 CSMUIR > 醫學院 > 護理學系暨碩士班 > 研究計劃 > Item 310902500/2652

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/2652

Title:	環境危險因子、代謝基因之多型性與子宮頸前癌和子宮頸癌之相關性探討 Environmental Hazards, Polymorphisms of Metabolizing Genes, and Cervical Intraepithelial Neoplasm Risk
Authors:	蔡秀婷 Tsai, Hsiu-Ting
Contributors:	中山醫學院護理學系
Date:	2008
Issue Date:	2010-11-05T10:08:04Z (UTC)
Abstract:	研究背景: 衛生署資料顯示，子宮頸癌為臺灣婦女癌症發生率的第一位，約每十萬位婦女，就有49.8 位罹患子宮頸癌。許多流行病學研究發現，高危險性人類乳突狀病毒感染、抽煙、暴露二手煙等，是導致婦女罹患子宮頸癌的重要因素。但是，有些個體雖暴露於以上的危險因素之中，但並未罹患子宮頸癌。因此，個人的基因型態在決定其是否罹患子宮頸癌上扮演重要角色。有研究指出phase II 酵素，例如GSTM1、 GSTT1、 GSTA1 及GSTP1 可將致癌物質轉換為親水性代謝物以排出體外。因此個體代謝致癌物質及去毒化的能力，可能會影響其是否會罹患癌症。本研究探討phase II 酵素，例如GSTM1、 GSTT1、 GSTA1 及GSTP1 基因多型性與子宮頸癌的相關性。研究方法:本研究至目前為止共收集127 位罹患子宮頸上皮內細胞瘤(cervical intraepithelium neoplasm，簡稱:CIN)以上的個案及340 位健康對照者，研究中以PCR-RFLP 探討基因與子宮頸上皮內細胞瘤的相關性，並以問卷調查方式收集社會人口學及環境危險因子等相關資料，以探討基因與基因，及基因與環境中危險因子與子宮頸癌的相關性。初步結果:本研究初步結果發現，個體具I/V 或V/V 型的GSTP1 基因多型性相較於I/I 型基因多型性者有3.37 倍(95% CI: 1.30-8.72)的危險性會罹患子宮頸上皮內細胞瘤。而其他基因的基因多型性，例如GSTM1、 GSTT1 及GSTA1 則與罹患子宮頸上皮內細胞瘤無顯著相關性。結論: 個體具I/V 或V/V 型的GSTP1 基因多型性可能與婦女罹患子宮頸上皮內細胞瘤有關。 Background: According to the report from Department of Health, cervical cancer (49.8 per 100,000 females) was the most prevalent malignancy in Taiwanese women. It was also the fifth leading cause of cancer deaths among women (8.3 per 100,000 females). Many epidemiologic researchers have found that high-risk HPV (human papillomavirus) infection, active cigarette smoking, passive smoking, reproductive condition, and sexual behavior were the major risk factors of cervical cancer. However, those factors can’t fully explain the entire pathogenesis of cervical cancer. Some individuals under the exposure to those risk factors didn’t develop cervical intraepithelial neoplasm (CIN), suggesting genetic components may play another pivotal role in cervical carcinogenesis. Phase II enzymes, such as GSTM1 (μ), GSTA1 (α), GSTT1 (θ), and GSTP1 (π) have been suggested as detoxifying genes because of their ability to regulate the conjugation of a wide range of xenobiotics, including environmental carcinogens, in order to excrete as hydrophilic metabolites. Individuals with different abilities to metabolize and detoxify carcinogens show different risks to develop cancer. Although a few studies investigated the association between metabolize enzyme gene polymorphism phase II GST, and the progression of cervical neoplasm, the results are still controversial. In this study, we conduct a case–control study to investigate the role of genetic susceptibility in the development of cervical neoplasm. Methods: Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) was used to measure gene polymorphisms in both healthy controls and CIN patients. Results: A significant different frequencies of GSTP1 genotypes was found between healthy controls and CIN patients. The adjusted odds ratios (AORs) with their 95% confidence intervals (CIs) for individuals with I/V or V/V of GSTP1 compared to individuals with I/I of GSTP1 was 3.37 fold risk (95%CI: 1.30-8.72) to induce CIN1, however, there was not a significant different frequency between healthy controls and individuals with ≥ CIN2. As well, no significant difference was found between healthy controls and cervical intraepithelial neoplasm patients among GSTM1, GSTT1, and GSTA1 gene polymorphism. Conclusions: Individuals with I/V or V/V of GSTP1 gene polymorphism is considered as a factor increasing the susceptibility of cervical intraepithelial neoplasm.
URI:	https://ir.csmu.edu.tw:8080/handle/310902500/2652
Appears in Collections:	[護理學系暨碩士班] 研究計劃

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