中山醫學大學機構典藏 CSMUIR:Item 310902500/25118
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 17939/22958 (78%)
造访人次 : 7373996      在线人数 : 277
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧：
  • 您可在西文检索词汇前后加上"双引号"，以获取较精准的检索结果
  • 若欲以作者姓名搜寻，建议至进阶搜寻限定作者字段，可获得较完整数据
    •  进阶搜寻


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://ir.csmu.edu.tw:8080/ir/handle/310902500/25118


    题名: Both STT3A- and STT3B-containing OST complexes are involved in the asparagine-linked glycosylation of human R-SPONDIN 3
    作者: Tsai, Chiung-Fang Chang ； Hui-Fang Tsai ； Chu-Chyn Ou ； Jen-Ning
    贡献者: 中山醫學大學
    日期: 2022-06-01
    上传时间: 2022-10-21T03:22:35Z (UTC)
    出版者: 研發處育成中心暨產學合作組
    摘要: Purpose: R-spondins (RSPOs) play a pivotal role in stem cell development by potentiating Wnt signaling. We previously studied the biogenesis of RSPOs and found that asparagine-linked glycan (N-glycan) at N137 on RSPO1 affects intracellular stability and secretion efficiency. In the present study, we focused on RSPO3 as it has multiple N-linked glycan chains. Methods: The N-glycosylation sites on RSPO3 were explored by site-directed mutagenesis and Western blot analysis. The identification of the N-glycosyltransferase(s) responsible for RSPO3 N-glycosylation was approached by specific siRNA knockdown. Results: In addition to N137, two glycosylation sites at positions N36 and N194 were identified. Elimination of glycosylation at these sites led to opposing effects on RSPO3 secretion. While abrogation of N-glycosylation at N36 impaired its secretion, loss of N-glycan at N194 enhanced its secretion. These results highlight the differential roles of N-glycan at these two positions in the secretion of RSPO3. Finally, we showed that N137 is an STT3A-dependent site and N-glycosylation at N36 and N194 is STT3B-dependent using specific siRNA knockdown. Conclusion: Based on the results, STT3A- and STT3B-associated OST complexes differentially glycosylate RSPO3 at multiple sites.
    URI: https://ir.csmu.edu.tw:8080/handle/310902500/25118
    關聯: 中山醫學雜誌 ； 33卷1期 (2022 / 06 / 01) ， P17 - 24
    显示于类别:[研發處] 期刊論文

    文件中的档案:

    档案 描述 大小格式浏览次数
    Both STT3A- and STT3B-containing OST complexes are involved in the asparagine-linked glycosylation o.pdf652KbAdobe PDF105检视/开启


    检视Licence

    SFX Query

    在CSMUIR中所有的数据项都受到原著作权保护.

    TAIR相关文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈