Validation of Enhancing Effects of Curcumin on Radiotherapy with F98/FGTGlioblastoma-Bearing Rat Model

doi:10.3390/ijms21124385

中山醫學大學機構典藏 CSMUIR > 中山醫學大學研究成果 > 期刊論文 > Item 310902500/24853

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/24853

Title:	Validation of Enhancing Effects of Curcumin on Radiotherapy with F98/FGTGlioblastoma-Bearing Rat Model
Authors:	Wang, WH;Shen, CY;Chien, YC;Chang, WS;Tsai, CW;Lin, YH;Hwang, JJ
Keywords:	curcumin;F98;FGT;glioblastoma;radiosensitizer
Date:	2020
Issue Date:	2022-08-09T08:09:03Z (UTC)
Publisher:	MDPI
Abstract:	Glioblastoma, the most common and aggressive brain tumor with low survival rate, is difficult to be cured by neurosurgery or radiotherapy. Mounting evidence has reported the anti-inflammatory and anticancer effects of curcumin on several types of cancer in preclinical studies and clinical trials. To our knowledge, there is no platform or system that could be used to effectively and real-timely evaluate the therapeutic efficacy of curcumin for glioblastoma multiforme (GBM). In this study, we constructed a lentivirus vector with triple-reporter genes (Fluc/GFP/tk) and transduced into rat F98 glioblastoma cells to establish an orthotopic F98/FGTglioma-bearing rat model. In the model, the therapeutic efficacies for curcumin alone, radiation alone, and their combination were evaluated via noninvasive bioluminescent imaging and overall survival measurements. At the cell level, curcumin is capable of causing a G2/M cell cycle arrest and sensitizing the F98 cells to radiation. In animal model, curcumin synergistically enhances the effects of radiotherapy on suppressing the growth of both transplanted glioma cells and in situ brain tumors, and extending the overall survival periods longer than those of curcumin alone and radiation alone treatments. In conclusion, we have demonstrated that curcumin may serve as a novel radiosensitizer to combine with radiotherapy using the triple-reporter F98/FGTanimal model for effective and simultaneous evaluation of therapeutic efficacy.
URI:	http://dx.doi.org/10.3390/ijms21124385 https://www.webofscience.com/wos/woscc/full-record/WOS:000549457900001 https://ir.csmu.edu.tw:8080/handle/310902500/24853
Relation:	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES ,2020 ,v21 ,issue 12
Appears in Collections:	[中山醫學大學研究成果] 期刊論文

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