Aqueous Ocimum gratissimum extract induces cell apoptosis in human hepatocellular carcinoma cells

doi:10.7150/ijms.39436

中山醫學大學機構典藏 CSMUIR > 中山醫學大學研究成果 > 期刊論文 > Item 310902500/24727

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/24727

Title:	Aqueous Ocimum gratissimum extract induces cell apoptosis in human hepatocellular carcinoma cells
Authors:	Huang, CC;Hwang, JM;Tsai, JH;Chen, JH;Lin, H;Lin, GJ;Yang, HL;Liu, JY;Yang, CY;Ye, JC
Keywords:	Ocimum gratissimum;hepatocellular carcinoma;cell cycle arrest
Date:	2020
Issue Date:	2022-08-09T08:07:02Z (UTC)
Publisher:	IVYSPRING INT PUBL
ISSN:	1449-1907
Abstract:	Treatment of advanced hepatocellular carcinoma (HCC) has exhibited a poor overall survival rate of only six to ten months, and the urgency of the development of more effective novel agents is ever present. In this line of research, we aimed to investigate the effects and inhibitive mechanisms of aqueous Ocimum gratissimum leaf extract (OGE), the extract of Ocimum gratissimum, which is commonly used as a therapeutic herb for its numerous pharmacological properties, on malignant HCC cells. Our results showed that OGE decreased the cell viability of HCC SK-Hep1 and HA22T cells in a dose-dependent manner (from 400 to 800 mu g/mL), while there is little effect on Chang liver cells. Moreover, cell-cycle analysis shows increased Sub-G1 cell count in SK-Hep1 and HA22T cells which is not observed in Chang liver cells. These findings raise suspicion that the OGE-induced cell death may be mediated through proteins that regulate cell cycle and apoptosis in SK-Hep1 and HA22T cells, and further experimentation revealed that OGE treatment resulted in a dose-dependent decrease in caspase 3 and PARP expressions and in CDK4 and p-ERK1/2expressions. Moreover, animal tests also exhibited decreased HCC tumor growth by OGE treatment. We therefore suggest that the inhibition of cell viability and tumor growth induced by OGE may be correlated to the alteration of apoptosis-related proteins.
URI:	http://dx.doi.org/10.7150/ijms.39436 https://www.webofscience.com/wos/woscc/full-record/WOS:000514851300008 https://ir.csmu.edu.tw:8080/handle/310902500/24727
Relation:	INTERNATIONAL JOURNAL OF MEDICAL SCIENCES ,2020 ,v17 ,issue 3 ,p338-346
Appears in Collections:	[中山醫學大學研究成果] 期刊論文

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