Caffeic Acid-coated Nanolayer on Mineral Trioxide Aggregate Potentiates the Host Immune Responses, Angiogenesis, and Odontogenesis

doi:10.1016/j.joen.2020.07.003

中山醫學大學機構典藏 CSMUIR > 中山醫學大學研究成果 > 期刊論文 > Item 310902500/24584

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/24584

Title:	Caffeic Acid-coated Nanolayer on Mineral Trioxide Aggregate Potentiates the Host Immune Responses, Angiogenesis, and Odontogenesis
Authors:	Tu, MG;Lee, AKX;Lin, YH;Huang, TH;Ho, CC;Shie, MY
Keywords:	Angiogenesis;caffeic acid;human dental pulp stem cell;inflammation;mineral trioxide aggregate;odontogenesis;RAW 264.7
Date:	2020
Issue Date:	2022-08-09T08:04:44Z (UTC)
Publisher:	ELSEVIER SCIENCE INC
ISSN:	0099-2399
Abstract:	Introduction: The aim of this study was to investigate whether mineral trioxide aggregate (MTA) can be modified with caffeic acid (CA) to form caffeic acid/mineral trioxide aggregate (CAMTA) cement and to evaluate its physicochemical and biological properties as well as its capability in immune suppression and angiogenesis. Methods: MTA was immersed in trishydroxymethyl aminomethane buffer with CA to allow coating onto MTA powders. X-ray diffractometry and tensile stress-strain tests were conducted to assess for physical characteristics of CAMTA and to evaluate for successful modification of MTA. Then, the CAMTA cement was immersed in simulated body fluid to evaluate its hydroxyapatite formation capabilities and Si release profiles. In addition, RAW 264.7 cells and human dental pulp stem cells were used to evaluate CAMTA's immunosuppressive capabilities and cell responses, respectively. hDPSCs were also used to assess CAMTA's angiogenic capabilities. Results: The X-ray diffractometry results showed that CA can be successfully coated onto MTA without disrupting or losing MTA's original structural properties, thus allowing us to retain the initial advantages of MTA. CAMTA was shown to have higher mechanical properties compared with MTA and had rougher pitted surfaces, which were hypothesized to lead to enhanced adhesion, proliferation, and secretion of angiogenic- and odontogenic-related proteins. In addition, it was found that CAMTA was able to enhance hydroxyapatite formation and immunosuppressive capabilities compared with MTA. Conclusions: CAMTA cements were found to have improved physicochemical and biological characteristics compared with their counterpart. In addition, CAMTA cements had enhanced odontogenic, angiogenic, and immunosuppressive properties compared with MTA. All of the results of this study proved that CAMTA cements could be a biomaterial for future clinical applications and tissue engineering use.
URI:	http://dx.doi.org/10.1016/j.joen.2020.07.003 https://www.webofscience.com/wos/woscc/full-record/WOS:000577972400009 https://ir.csmu.edu.tw:8080/handle/310902500/24584
Relation:	JOURNAL OF ENDODONTICS ,2020 ,v46 ,issue 10 ,p1455-1464
Appears in Collections:	[中山醫學大學研究成果] 期刊論文

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