Protective Role of Vernonia Amygdalina Leaf Aqueous Extract Against Neomycin-Induced Hair Cell Damage in Transgenic Zebrafish

doi:10.37290/ctnr2641-452X.18:366-372

中山醫學大學機構典藏 CSMUIR > 中山醫學大學研究成果 > 期刊論文 > Item 310902500/24502

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/24502

Title:	Protective Role of Vernonia Amygdalina Leaf Aqueous Extract Against Neomycin-Induced Hair Cell Damage in Transgenic Zebrafish
Authors:	Hsu, CF;Yang, FA;Liu, SC;Li, SY;Song, HW;Liang, ZC;Yang, JJ
Keywords:	Animal model;Antioxidant;Hair cell;Ototoxicity;Vemonia amygdalina
Date:	2020
Issue Date:	2022-08-09T08:03:23Z (UTC)
Publisher:	NEW CENTURY HEALTH PUBLISHERS, LLC
ISSN:	1540-7535
Abstract:	Aminoglycoside treatment increases the loss of hair cells in the inner ear. Once the hair cells are lost, they are unable to regenerate spontaneously, and the resulting hearing loss is profound and irreversible in humans. The aqueous extract from the leaves of Vernonia amygdalina has been reported to have antioxidant properties. In this study, we evaluated whether the aqueous extracts of Vernonia amygdalina leaf could protect hair cells from aminoglycoside-induced damage using a transgenic zebrafish line. Our results showed that the EC50 of 1,1-Diphenyl-2 picrylhydrazyl radical scavenging and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulphonate), a measure of antioxidant capacity, of the aqueous extracts of Vernonia amygdalina leaf was 11.31 mg/mL and 2.73 mg/mL, respectively. Moreover, the total phenolic and flavonoids contents were 11.48 +/- 3.12 mg gallic acid/g and 6.89 +/- 0.65 mg catechin/g, respectively. In the toxicology study, the larval survival rate was significantly decreased and was dependent on the dosage of aqueous extracts of Vernonia amygdalina leaf, whereas it had no significant toxicity at doses below 2.5 mg/mL concentration (survival rate= 100%). Furthermore, a hair cell survival study shows that aqueous extracts of Vernonia amygdalina leaf decreased neomycin-induced hair cell death in a dose-dependent manner (survival rate = similar to 94.1 +/- 3.1% at 2 mg/mL concentration). Overall, these results suggest that the aqueous extracts of Vernonia amygdalina leaf possess the antioxidant effect and protects against hair cell loss caused by toxic side effects of neomycin. An optimal aqueous extract of Vernonia amygdalina leaf can be consumed as an herbal medicine without probable toxicity to body tissues and organs.
URI:	http://dx.doi.org/10.37290/ctnr2641-452X.18:366-372 https://www.webofscience.com/wos/woscc/full-record/WOS:000559732800012 https://ir.csmu.edu.tw:8080/handle/310902500/24502
Relation:	CURRENT TOPICS IN NUTRACEUTICAL RESEARCH ,2020 ,v18 ,issue 4 ,p366-372
Appears in Collections:	[中山醫學大學研究成果] 期刊論文

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